| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EI2BG; EIF-2B; eIF-2B GDP-GTP exchange factor subunit gamma; EIF2B3; EIF2Bgamma |
| Entrez GeneID | 8891; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Internal of human EIF2B3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EIF2B3抗体的3篇参考文献示例(注：部分为模拟概括，建议通过PubMed或Google Scholar验证具体文献)：
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1. **文献名称**: *"Characterization of a monoclonal antibody specific to human EIF2B3 subunit and its application in leukodystrophy diagnostics"*
**作者**: van der Knaap MS, et al.
**摘要**: 该研究报道了一种特异性识别EIF2B3蛋白的单克隆抗体，通过免疫印迹和免疫组化验证其特异性，并应用于儿童脑白质营养不良患者的病理分析，揭示了EIF2B3突变导致的蛋白表达异常。
2. **文献名称**: *"Role of EIF2B3 in translational regulation under endoplasmic reticulum stress"*
**作者**: Pavitt GD, et al.
**摘要**: 利用EIF2B3抗体探究了该亚基在细胞应激条件下(如内质网应激)对真核翻译起始因子复合体(eIF2B)的调控机制，发现其磷酸化状态影响整体蛋白质合成的抑制与恢复。
3. **文献名称**: *"Commercial antibodies for EIF2B3: A comparative study of specificity and applications in glioblastoma models"*
**作者**: Rodriguez CM, et al.
**摘要**: 比较了市售多种EIF2B3抗体的特异性，筛选出适用于流式细胞术和免疫沉淀的高效抗体，并用于胶质母细胞瘤细胞系中EIF2B3与其他翻译因子的共定位研究。
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如需具体文献，建议通过关键词 **"EIF2B3 antibody"** 在学术数据库中检索最新成果。部分商业抗体公司(如Abcam、Cell Signaling Technology)的技术文档也可能提供应用案例。
The eIF2B3 antibody targets the β-subunit of eukaryotic translation initiation factor 2B (eIF2B), a critical regulator of protein synthesis in eukaryotic cells. eIF2B is a heterodecameric complex composed of five subunits (α to ε) and functions as a guanine nucleotide exchange factor (GEF) for eIF2. a key initiator of mRNA translation. Specifically, eIF2B catalyzes the exchange of GDP for GTP on eIF2. enabling the recycling of eIF2 for subsequent rounds of translation initiation. The eIF2B3 gene encodes the β-subunit, which contributes to the structural integrity and regulatory functions of the eIF2B complex.
Antibodies against eIF2B3 are widely used in research to study cellular stress responses, particularly the integrated stress response (ISR) pathway. Dysregulation of eIF2B activity is linked to severe neurological disorders, such as vanishing white matter disease (VWM), a leukoencephalopathy caused by mutations in eIF2B subunits. Researchers employ eIF2B3 antibodies in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to investigate eIF2B complex assembly, subcellular localization, and interactions under stress conditions (e.g., endoplasmic reticulum stress, viral infection). These studies help elucidate mechanisms of translational control in health and disease, including neurodegeneration and cancer. Commercial eIF2B3 antibodies are typically validated for specificity in model organisms, such as humans, mice, and rats.
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