| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C14orf20; EC 2.7.11.1; serine/threonine-protein kinase 22E; STK22E; testis-specific kinase 4 |
| Entrez GeneID | 283629; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human TSSK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TSSK4抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Characterization of TSSK4 expression and its role in spermatogenesis using a specific polyclonal antibody"*
**作者**：Li Y, et al.
**摘要**：本研究通过免疫兔子生成了一种针对人类TSSK4蛋白C末端的多克隆抗体，验证了其在睾丸组织中的特异性表达。免疫组化显示TSSK4主要在精子细胞的顶体和尾部定位，提示其可能参与精子形态建成和成熟过程。
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2. **文献名称**：*"TSSK4 deficiency disrupts acrosome formation and leads to male infertility in mice"*
**作者**：Wang X, et al.
**摘要**：作者利用CRISPR-Cas9技术构建TSSK4敲除小鼠模型，并采用Western blot和免疫荧光技术(使用商业化TSSK4抗体)分析蛋白缺失表型。结果显示，TSSK4缺失导致顶体发育异常和精子活力下降，证实其对男性生育能力的关键作用。
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3. **文献名称**：*"Development of a monoclonal antibody against TSSK4 for detecting testicular germ cell tumors"*
**作者**：Zhang R, et al.
**摘要**：该研究开发了一种高特异性抗TSSK4单克隆抗体，通过ELISA和免疫组化验证其在睾丸生殖细胞肿瘤(TGCT)组织中的高表达。结果表明，TSSK4可能作为TGCT的生物标志物，抗体在临床诊断中具有潜在应用价值。
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**备注**：以上文献为示例性质，实际文献需通过PubMed或Web of Science检索获取。建议使用关键词“TSSK4 antibody”或“TSSK4 kinase”筛选近年研究。
The TSSK4 (testis-specific serine kinase 4) antibody is a research tool designed to detect and study the TSSK4 protein, a member of the testis-specific serine/threonine kinase family. TSSK kinases are predominantly expressed in the testis and are implicated in spermatogenesis and male fertility. TSSK4. encoded by the *TSSK4* gene in humans, shares structural features with other family members, including a conserved kinase domain. Its specific expression in post-meiotic germ cells suggests a role in late-stage sperm development, such as sperm maturation, motility, or acrosome formation.
Research using TSSK4 antibodies has helped elucidate its localization, expression patterns, and interactions with proteins like TSKS (testis-specific kinase substrate). Studies in knockout models indicate that TSSK4 deficiency disrupts spermiogenesis, leading to male infertility. These findings highlight its potential as a target for male contraception or infertility treatments.
Commercially available TSSK4 antibodies are typically developed in rabbits or mice using immunogenic peptide regions. They are validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. However, specificity and batch variability require careful validation due to homology among TSSK family members. Ongoing research explores TSSK4's broader roles, including possible ectopic expression in cancers, expanding its relevance beyond reproductive biology.
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