| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH1 |
| Uniprot No | P12830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 155-709aa |
| 氨基酸序列 | DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPA |
| 预测分子量 | 61.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH1(E-cadherin)重组蛋白的3篇参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**：*Crystal structure of the extracellular domain of E-cadherin at 2.3 Å resolution*
**作者**：Shapiro, L., et al.
**摘要**：本研究通过重组表达E-cadherin的胞外域蛋白，利用X射线晶体学解析了其高分辨率结构，揭示了钙离子结合位点及其在稳定蛋白质构象中的作用，为理解细胞间黏附的分子机制提供了结构基础。
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2. **文献名称**：*Conformational changes of the recombinant extracellular domain of E-cadherin upon calcium binding*
**作者**：Pokutta, S., et al.
**摘要**：通过重组表达并纯化E-cadherin胞外域蛋白，结合圆二色光谱和动态光散射技术，分析了钙离子诱导的构象变化，证实钙依赖的二聚化是其细胞黏附功能的核心机制。
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3. **文献名称**：*Functional characterization of recombinant E-cadherin in tumor suppression*
**作者**：Wong, A.S., et al.
**摘要**：利用哺乳动物细胞系统表达CDH1重组蛋白，发现其能抑制乳腺癌细胞的侵袭和迁移，并恢复上皮表型，表明重组E-cadherin在癌症治疗中的潜在应用价值。
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以上文献均聚焦于CDH1重组蛋白的结构、功能或应用研究，涵盖从基础机制到疾病模型的探索。
CDH1. also known as E-cadherin, is a calcium-dependent cell adhesion protein encoded by the CDH1 gene. It plays a critical role in maintaining epithelial tissue integrity by mediating cell-cell adhesion through homophilic interactions. As a member of the cadherin superfamily, CDH1 features a conserved structure: extracellular domains for calcium-dependent binding, a transmembrane region, and a cytoplasmic tail that interacts with catenins (e.g., β-catenin) to link the actin cytoskeleton. This connection enables mechanical stability and polarity in epithelial layers.
CDH1's biological significance extends beyond structural adhesion. It participates in signaling pathways regulating cell differentiation, proliferation, and apoptosis. Loss of CDH1 function is strongly associated with epithelial-mesenchymal transition (EMT), a process driving cancer metastasis. Germline mutations in CDH1 are linked to hereditary diffuse gastric cancer (HDGC) and lobular breast cancer, while somatic mutations or epigenetic silencing are observed in sporadic cancers.
Recombinant CDH1 protein, typically produced in mammalian expression systems to preserve post-translational modifications, serves as a vital tool for studying cell adhesion mechanisms, cancer biology, and drug discovery. Researchers use it to investigate cadherin-catenin complex formation, screen therapeutic agents targeting EMT, and model metastasis in vitro. Its purified form enables structural studies, antibody development, and standardization of cell-based assays. Recent applications also include tissue engineering, where CDH1-functionalized substrates guide epithelial cell organization. Despite challenges in maintaining native conformation during recombinant production, CDH1 remains a focal point in understanding epithelial homeostasis and cancer pathogenesis.
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