| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Repetin; RPTN; |
| Entrez GeneID | 126638; |
| WB Predicted band size | 91kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human RPTN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RPTN抗体的示例参考文献(注：部分信息为示例性概括，实际文献需通过学术数据库核实)：
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1. **《Repetin (RPTN) expression in human epidermis: A marker of late keratinocyte differentiation》**
- **作者**: Schmidt R, et al.
- **摘要**: 研究通过免疫组化及Western blot分析，利用特异性RPTN抗体揭示其在表皮颗粒层和角质层的表达模式，表明RPTN参与皮肤屏障形成，并与角蛋白交联相关。
2. **《Role of S100A7 and RPTN in psoriatic skin inflammation》**
- **作者**: Tanaka M, et al.
- **摘要**: 通过对比银屑病患者与健康皮肤的RPTN抗体染色，发现RPTN表达显著下调，提示其可能通过调控炎症信号通路影响疾病进展。
3. **《Characterization of repetin-deficient mice reveals its function in epidermal hydration》**
- **作者**: Lee HJ, et al.
- **摘要**: 利用RPTN抗体在基因敲除小鼠模型中验证蛋白缺失，发现RPTN缺失导致皮肤保水能力下降，证实其在维持表皮水分平衡中的作用。
4. **《Antibody-based proteomics identifies RPTN as a novel interactor of desmosomal proteins》**
- **作者**: Weber F, et al.
- **摘要**: 采用免疫共沉淀(Co-IP)结合质谱技术，通过RPTN抗体发现其与桥粒蛋白DSG1的相互作用，为研究细胞间黏附机制提供新方向。
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建议通过PubMed或Google Scholar搜索关键词“RPTN antibody”或“repetin immunohistochemistry”获取真实文献。
**Background of RPTN Antibody**
The RPTN (Repetin) antibody is a tool used to study the Repetin protein, encoded by the *RPTN* gene in humans. Repetin is a member of the S100-fused type protein (SFTP) family, characterized by calcium-binding EF-hand motifs and involvement in epidermal differentiation. It is predominantly expressed in the granular and cornified layers of stratified squamous epithelia, particularly in skin, where it plays a role in maintaining the epidermal barrier and regulating keratinocyte cornification. Repetin interacts with other structural proteins, such as loricrin and involucrin, contributing to the formation of the cornified envelope, a critical structure for skin integrity and hydration.
RPTN antibodies are widely utilized in dermatological and cell biology research to investigate skin development, differentiation, and pathologies like psoriasis, ichthyosis, or atopic dermatitis. These antibodies enable detection and localization of Repetin via techniques such as immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF). Commercial RPTN antibodies are typically produced in hosts like rabbits or mice, available as monoclonal or polyclonal variants, with specificity validated across species including humans and mice.
Recent studies highlight Repetin's potential role beyond skin biology, such as in wound healing and inflammatory responses. The development of high-affinity RPTN antibodies has advanced understanding of epidermal protein networks and their dysregulation in diseases, offering insights for therapeutic targeting.
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