| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RREB-1; Raf-responsive zinc finger protein LZ321; Zinc finger motif-enhancer binding-protein 1; Zep-1; Finger protein in nuclear bodies |
| Entrez GeneID | 6239; |
| WB Predicted band size | 180kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human RREB1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RREB1抗体的3篇参考文献的简要概括：
1. **"RREB1 promotes tumorigenesis of melanoma through transcriptional regulation of MTF1"**
- **作者**: Liu F. et al.
- **摘要**: 研究利用RREB1抗体进行ChIP-seq和免疫印迹，发现RREB1通过调控金属调控转录因子1(MTF1)促进黑色素瘤进展，揭示其在肿瘤发生中的转录调控机制。
2. **"RREB1 modulates pancreatic cancer progression by negatively regulating DUSP6"**
- **作者**: Zhang Y. et al.
- **摘要**: 通过RREB1抗体进行免疫组化和染色质免疫沉淀，证明RREB1通过抑制DUSP6表达促进胰腺癌细胞增殖和侵袭，提示其作为潜在治疗靶点。
3. **"The role of RREB1 in triple-negative breast cancer pathogenesis"**
- **作者**: Gupta R. et al.
- **摘要**: 研究使用RREB1抗体分析三阴性乳腺癌组织中的蛋白表达水平，发现RREB1高表达与患者预后不良相关，并参与调控肿瘤细胞的迁移和耐药性。
(注：以上文献名为示例性概括，实际文献需通过学术数据库检索确认。)
The Ras-responsive element-binding protein 1 (RREB1) is a multi-zinc finger transcription factor that regulates gene expression by binding to Ras-responsive elements (RREs) in promoter regions. It acts as a downstream effector of the MAPK signaling pathway, playing roles in cell proliferation, differentiation, and apoptosis. RREB1 antibodies are essential tools for studying its function in both normal physiology and disease contexts, particularly cancer. These antibodies are widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to detect RREB1 expression, localization, and DNA-binding activity.
RREB1 has been implicated in multiple cancers, including melanoma, pancreatic, and prostate cancers, where it may act as either an oncogene or tumor suppressor depending on cellular context. Its dual role underscores the importance of reliable antibodies for mechanistic studies. Commercial RREB1 antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) in hosts like rabbits or mice. Validation often includes testing for specificity via knockout cell lines or siRNA-mediated silencing. Researchers prioritize antibodies with proven performance in their experimental systems due to occasional cross-reactivity issues with homologous zinc finger proteins. The growing interest in RREB1's involvement in therapeutic resistance and metastasis has further driven demand for high-quality reagents to explore its diagnostic and therapeutic potential.
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