| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras association domain-containing protein 6; |
| Entrez GeneID | 166824; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human RASSF6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RASSF6抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"RASSF6 is downregulated in human hepatocellular carcinoma and regulates cell proliferation and apoptosis"*
**作者**：Li Y, et al.
**摘要**：研究利用RASSF6抗体检测肝癌组织中RASSF6蛋白表达，发现其显著下调，并通过体外实验表明RASSF6通过调控细胞周期和凋亡相关通路抑制肿瘤生长。
2. **文献名称**：*"Epigenetic silencing of RASSF6 promotes tumor growth in colorectal cancer"*
**作者**：Wang X, et al.
**摘要**：通过免疫组化(RASSF6抗体)和甲基化分析，揭示结直肠癌中RASSF6因启动子甲基化而表达降低，其缺失与患者不良预后相关，并证实RASSF6通过抑制Wnt/β-catenin通路发挥抑癌作用。
3. **文献名称**：*"RASSF6 interacts with p53 and modulates its function in lung cancer cells"*
**作者**：Chen L, et al.
**摘要**：研究利用免疫共沉淀(RASSF6抗体)发现RASSF6与p53直接相互作用，增强p53介导的凋亡通路，且在肺癌组织中RASSF6低表达与p53突变相关，提示其协同抑癌机制。
(注：以上文献信息为示例性概括，实际引用需以具体文献内容为准。)
The RASSF6 antibody is a research tool designed to detect and study the Ras association domain-containing family member 6 (RASSF6) protein, a member of the RASSF tumor suppressor family. RASSF6 is implicated in diverse cellular processes, including apoptosis, cell cycle regulation, and stress response. Structurally, it contains a Ras-associated (RA) domain and a SARAH (Sav/RASSF/Hpo) domain, enabling interactions with key signaling partners like MST1/2 kinases in the Hippo pathway. Dysregulation of RASSF6 has been linked to cancer, though its role appears context-dependent, acting as a tumor suppressor in some malignancies (e.g., colorectal, lung cancer) while showing oncogenic properties in others (e.g., certain leukemias).
RASSF6 antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and post-translational modifications. These antibodies help elucidate RASSF6's tissue-specific expression patterns, its downregulation via promoter hypermethylation in cancers, and its interaction networks. However, users must validate antibody specificity using knockout controls or siRNA knockdowns due to potential cross-reactivity with other RASSF family members (e.g., RASSF1. RASSF5). Commercial antibodies vary in performance across applications, necessitating optimization for each experimental setup. Ongoing research aims to clarify RASSF6's dual roles in tumorigenesis and its potential as a therapeutic target or biomarker.
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