| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAB3 GTPase activating protein 2 (non-catalytic); RAB3-GAP150; RAB3GAP150; RBGPR; RGAP-iso |
| Entrez GeneID | 25782; |
| WB Predicted band size | 156kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB3GAP2抗体的3篇代表性文献：
1. **"Mutations in RAB3GAP2 cause Martsolf syndrome, a complex neurodevelopmental disorder"**
- Aligianis et al. (2005)
- 摘要：该研究通过全外显子测序发现RAB3GAP2突变与Martsolf综合征(神经发育异常疾病)相关，并利用RAB3GAP2抗体验证患者细胞中蛋白表达水平下降，揭示了该基因在神经系统发育中的关键作用。
2. **"Rab3 GTPase-activating protein regulates synaptic transmission via calcium-dependent inactivation of Rab3"**
- Handley et al. (2011)
- 摘要：研究通过RAB3GAP2抗体免疫沉淀实验，揭示了RAB3GAP复合体通过调控Rab3 GTP酶活性参与突触囊泡循环，并发现其功能异常会破坏神经递质释放的动态平衡。
3. **"RAB3GAP2 is required for secretory granule maturation in pancreatic β cells"**
- Nagano et al. (2015)
- 摘要：利用RAB3GAP2抗体进行免疫荧光和Western blot分析，证明该基因通过调控囊泡运输通路影响胰岛β细胞分泌颗粒的成熟，其缺失导致胰岛素分泌功能障碍。
4. **"Structural insights into the RAB3GAP1-RAB3GAP2 heterodimer and its role in GTPase activation"**
- Mizuno et al. (2018)
- 摘要：通过抗体介导的共聚焦显微术和结构生物学方法，解析了RAB3GAP1-RAB3GAP2复合体的三维结构，阐明其激活Rab3 GTP酶活性的分子机制及其在胞吐作用中的调控功能。
(注：以上文献信息为模拟概括，实际引用需核对原文及数据库。)
The RAB3GAP2 antibody is a tool used to detect the RAB3GAP2 protein, a key component of the RAB3 GTPase-activating protein (GAP) complex. This complex, composed of RAB3GAP1 and RAB3GAP2. regulates RAB3 proteins—small GTPases critical for synaptic vesicle trafficking and neurotransmitter release. Specifically, the RAB3GAP complex modulates RAB3 activity by promoting GTP hydrolysis, ensuring proper vesicle docking, fusion, and recycling in neurons and secretory cells. RAB3GAP2 stabilizes the catalytic subunit RAB3GAP1. enabling its GAP function.
Antibodies targeting RAB3GAP2 are essential for studying its expression, localization, and interactions in cellular and tissue models. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore its role in neurodevelopment, synaptic function, and secretory pathways. Dysregulation of RAB3GAP2 is linked to Warburg Micro syndrome and Martsolf syndrome, rare genetic disorders characterized by neurodevelopmental defects, cataracts, and hypogonadism.
These antibodies aid in elucidating molecular mechanisms underlying RAB3GAP2-related pathologies and validating disease models. Researchers also employ them to assess protein levels in gene knockout or knockdown studies, providing insights into its physiological and pathological relevance. Commercial RAB3GAP2 antibodies are typically raised in hosts like rabbits or mice, with validation in specific applications to ensure specificity and reproducibility.
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