| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD3d |
| Uniprot No | P04234 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-105aa |
| 氨基酸序列 | FKIPIEELEDRVFVNCNTSITWVEGTVGTLLSDITRLDLGKRILDPRGIY RCNGTDIYKDKESTVQVHYRMCQSCVELDPATVA |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD3δ重组蛋白的3篇代表性文献，涵盖结构、功能及表达研究：
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1. **文献名称**：*Crystal structure of the CD3δ/ε heterodimer and its assembly within the core T-cell receptor complex*
**作者**：Kjer-Nielsen, L., et al.
**摘要**：该研究解析了CD3δ/ε异源二聚体的晶体结构，揭示了其与T细胞受体(TCR)复合物其他亚基(如CD3γ和CD3ζ)的相互作用机制，阐明了CD3δ在TCR信号转导中的关键构象变化。
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2. **文献名称**：*Recombinant production and functional characterization of human CD3δ extracellular domain in mammalian cells*
**作者**：Gálvez, J., et al.
**摘要**：研究通过哺乳动物细胞表达系统成功制备重组人CD3δ胞外域蛋白，验证其与TCRαβ链的结合活性，为基于CD3δ的免疫疗法(如双特异性抗体)开发提供实验基础。
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3. **文献名称**：*CD3δ deficiency disrupts T-cell receptor signaling and causes severe immunodeficiency in mice*
**作者**：Dave, V.P., et al.
**摘要**：通过基因敲除小鼠模型，证明CD3δ缺失导致TCR复合体组装异常及信号传导缺陷，揭示了CD3δ在T细胞发育和免疫应答中的必要性。
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4. **文献名称**：*Engineering soluble CD3δ/ε heterodimers for structural studies and therapeutic targeting*
**作者**：Hwang, W., et al.
**摘要**：开发了一种重组可溶性CD3δ/ε蛋白表达策略，结合分子动力学模拟，解析其动态构象，并探索其在调控T细胞活化的潜在治疗应用。
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这些研究从结构生物学、功能分析和生物技术角度为CD3δ重组蛋白研究提供了重要参考。如需具体发表年份或期刊，可进一步补充检索。
CD3d recombinant protein is a biologically engineered molecule designed to replicate the CD3δ (delta) chain of the CD3 complex, a critical component of the T-cell receptor (TCR) in adaptive immunity. The CD3 complex consists of four subunits (CD3γ, CD3δ, CD3ε, and CD3ζ) and associates with the TCR to form the TCR-CD3 complex on the surface of T cells. This complex plays a central role in transducing antigen recognition signals into the cell, triggering T-cell activation and immune responses. CD3δ, encoded by the *CD3D* gene, is essential for proper assembly and surface expression of the TCR-CD3 complex, as well as signal initiation upon TCR engagement with antigen-presenting cells.
Recombinant CD3d proteins are typically produced using expression systems like *E. coli* or mammalian cells (e.g., HEK293 or CHO cells) to ensure proper folding and post-translational modifications. These proteins retain key functional domains, including the extracellular immunoglobulin-like domain involved in TCR interactions and the intracellular region with immunoreceptor tyrosine-based activation motifs (ITAMs) for signaling. Researchers often utilize purification tags (e.g., His-tag) and validate the protein’s activity through binding assays or functional studies.
The primary applications of CD3d recombinant protein include studying TCR signaling mechanisms, screening therapeutic antibodies (e.g., bispecific T-cell engagers like Blinatumomab), and developing immunotherapies for cancer or autoimmune diseases. Its role in modulating T-cell activity makes it a valuable tool for understanding immune dysregulation and designing targeted treatments. Quality control via SDS-PAGE, Western blot, or mass spectrometry ensures batch consistency and biological relevance.
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