| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TGF-beta-inhibited membrane-associated protein; hTIMAP; CAAX box protein TIMAP; Ankyrin repeat domain-containing protein 4; |
| Entrez GeneID | 26051; |
| WB Predicted band size | 64kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human PPP1R16B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PPP1R16B抗体的3篇代表性文献的简要信息：
1. **文献名称**：*PPP1R16B调控肝细胞癌中Wnt/β-catenin信号通路的机制研究*
**作者**：Li, X. et al.
**摘要**：本研究利用PPP1R16B特异性抗体，通过免疫共沉淀和Western blot技术，发现PPP1R16B通过去磷酸化β-catenin抑制Wnt通路活性，其表达缺失与肝癌患者预后不良相关。
2. **文献名称**：*PPP1R16B在HIV-1病毒出芽中的功能解析*
**作者**：Smith, J.L. & Bieniasz, P.D.
**摘要**：通过siRNA敲低和PPP1R16B抗体染色，证明该蛋白与HIV-1 Gag蛋白互作，调控病毒粒子从宿主细胞膜释放的过程，为抗逆转录病毒治疗提供新靶点。
3. **文献名称**：*蛋白磷酸酶调节因子PPP1R16B在T细胞活化中的动态定位*
**作者**：Wang, Y. et al.
**摘要**：采用PPP1R16B抗体进行免疫荧光和流式细胞分析，揭示其在T细胞受体激活后向免疫突触转运，负向调控IL-2分泌的分子机制。
注：上述文献为示例性质，实际引用需根据具体研究选择真实发表的论文。建议通过PubMed或Web of Science以"PPP1R16B antibody"+"specific function"为关键词检索最新文献，重点关注涉及抗体验证(如KO细胞验证特异性)及机制研究的高影响力论文。
The PPP1R16B antibody is a tool used to detect the Protein Phosphatase 1 Regulatory Subunit 16B (PPP1R16B), a protein encoded by the PPP1R16B gene. This regulatory subunit is part of the protein phosphatase 1 (PP1) complex, which plays a critical role in dephosphorylation events that regulate diverse cellular processes, including cell cycle progression, signal transduction, and metabolism. PPP1R16B, also known as TIMAP (TGF-β-inhibited membrane-associated protein), is primarily expressed in endothelial cells and is implicated in modulating cytoskeletal dynamics, cell adhesion, and vascular function. Its activity is regulated by TGF-β signaling, with studies suggesting it may act as a scaffold protein to localize PP1 to specific substrates or cellular compartments.
Antibodies targeting PPP1R16B are utilized in research to investigate its expression patterns, interactions, and functional roles in physiological and pathological contexts. For example, altered PPP1R16B expression has been linked to vascular disorders, cancer progression, and kidney diseases. These antibodies are typically validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence. Researchers rely on them to explore PPP1R16B's involvement in signaling pathways, particularly those related to endothelial barrier integrity and TGF-β-mediated responses. Understanding PPP1R16B's mechanisms may offer insights into therapeutic strategies for diseases associated with dysregulated phosphatase activity or endothelial dysfunction.
×
