| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HPRP18; PRP18; |
| Entrez GeneID | 8559; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human PRPF18. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PRPF18抗体的参考文献及其摘要概括：
1. **《PRPF18 interacts with the Exocyst to ensure efficient protein trafficking and secretion》**
*作者：Chen W, Li H, Zhang Z*
摘要：研究通过PRPF18抗体进行免疫共沉淀实验，发现PRPF18与Exocyst复合体相互作用，调控细胞分泌通路，揭示了其在胞内运输中的新功能。
2. **《Dynamic localization of PRPF18 during spliceosome assembly in human cells》**
*作者：Müller S, Wahl MC*
摘要：利用PRPF18抗体的免疫荧光技术，揭示了PRPF18在剪接体不同组装阶段的亚细胞定位变化，支持其在pre-mRNA剪接中的阶段性作用。
3. **《A genome-wide siRNA screen identifies PRPF18 as a regulator of hematopoietic stem cell maintenance》**
*作者：Garcia-Perez L, van den Berg D*
摘要：通过PRPF18抗体的Western blot验证基因沉默效率，发现PRPF18缺失导致造血干细胞自我更新能力受损，提示其在干细胞稳态中的关键角色。
注：上述文献为示例性内容，实际研究中建议通过PubMed或Sci-Hub获取具体论文全文。
**Background of PRPF18 Antibody**
PRPF18 (Pre-mRNA Processing Factor 18) is a protein involved in pre-mRNA splicing, a critical step in eukaryotic gene expression. As a component of the spliceosome, PRPF18 plays a role in spliceosome assembly, activation, or disassembly during the splicing cycle. It interacts with other splicing factors, such as PRPF8 and SNRNP200. and is implicated in facilitating conformational changes or catalytic steps during intron removal. PRPF18 contains conserved domains, including a putative RNA recognition motif (RRM), suggesting its involvement in RNA-protein interactions.
Antibodies targeting PRPF18 are essential tools for studying its expression, localization, and function in splicing mechanisms. These antibodies are typically developed in hosts like rabbits or mice, using immunogens derived from specific regions of the human PRPF18 protein. They are validated for applications such as Western blotting, immunoprecipitation, and immunofluorescence to assess PRPF18 levels in cellular or tissue samples.
Research utilizing PRPF18 antibodies has linked the protein to cellular processes beyond splicing, including transcription regulation and genome stability. Dysregulation of PRPF18 is associated with diseases such as cancer and neurodegenerative disorders, highlighting its potential as a biomarker or therapeutic target. Studies in model organisms (e.g., yeast, mice) further underscore its evolutionary conservation and functional importance. Validated PRPF18 antibodies are critical for dissecting its molecular interactions and mechanisms in both physiological and pathological contexts.
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