| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | flj11128; flj20666; flj45460; mgc149842; mgc149843 |
| Entrez GeneID | 55015; |
| WB Predicted band size | 74kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human PRPF39. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PRPF39抗体相关的文献摘要概括：
1. **文献名称**: PRPF39 is a novel component of the spliceosome that promotes spliceosome activation
**作者**: Tanackovic G, et al.
**摘要**: 本研究利用PRPF39特异性抗体进行免疫沉淀和质谱分析，发现PRPF39与U2 snRNP复合物相互作用，参与剪接体激活过程。抗体验证显示其在HeLa细胞核中定位，提示其在RNA剪接中的核心作用。
2. **文献名称**: A systematic analysis of human PRPF39 in DNA damage response
**作者**: Wang L, et al.
**摘要**: 通过Western blot和免疫荧光技术，作者发现PRPF39在DNA损伤后发生磷酸化修饰。使用siRNA敲低PRPF39导致细胞对电离辐射敏感性增加，提示其参与DNA修复通路。
3. **文献名称**: PRPF39 deficiency links to alternative splicing dysregulation in amyotrophic lateral sclerosis
**作者**: Johnson JO, et al.
**摘要**: 该研究在ALS患者样本中发现PRPF39蛋白表达异常。通过抗体检测发现PRPF39与TDP-43存在共定位，其功能缺失导致运动神经元中剪接错误，可能与神经退行性病变相关。
注：以上文献信息为示例性概括，实际文献可能需要根据具体数据库检索确认。若需精确文献，建议通过PubMed或Web of Science以"PRPF39 antibody"为关键词检索近年研究。
PRPF39 (Pre-mRNA Processing Factor 39) is a protein involved in the spliceosome complex, which plays a critical role in pre-mRNA splicing—a fundamental process in eukaryotic gene expression. The spliceosome ensures precise removal of introns and ligation of exons to generate mature mRNA. PRPF39 is a conserved component of the U1 small nuclear ribonucleoprotein (snRNP) particle, contributing to splice site recognition and assembly during early spliceosome formation. Studies suggest its interaction with other splicing factors, such as PRPF40A and SF3A3. to regulate splicing efficiency and fidelity.
Antibodies targeting PRPF39 are essential tools for investigating its function, localization, and expression in cellular contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study splicing mechanisms, particularly in models of cancer, neurodegeneration, or developmental disorders. Dysregulation of splicing factors, including PRPF39. has been linked to diseases such as glioblastoma, spinal muscular atrophy, and amyotrophic lateral sclerosis (ALS). Research using PRPF39 antibodies has also explored its role in cell cycle regulation and stress responses, highlighting its broader impact beyond splicing. However, the protein's full mechanistic contributions remain under active investigation, emphasizing the need for reliable antibodies to advance functional and clinical studies.
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