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Rabbit Polyclonal SF3B14 Antibody

  • 中文名: SF3B14抗体
  • 别    名: CGI-110; HSPC175; Ht006; PM14; pre-mRNA branch site protein p14
货号: IPDX42526
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCGI-110; HSPC175; Ht006; PM14; pre-mRNA branch site protein p14
Entrez GeneID51639;
WB Predicted band size20kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenSynthesized peptide derived from C-terminal of human SF3B14.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是基于现有知识模拟生成的关于SF3B14抗体的参考文献示例。由于SF3B14的研究可能较少,部分内容为假设性概括,建议通过学术数据库进一步验证:

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1. **文献名称**:*SF3B14 modulates pre-mRNA splicing through interaction with the U2 snRNP complex*

**作者**:Chen L, Wang Y, et al.

**摘要**:本研究利用特异性SF3B14抗体进行免疫共沉淀实验,揭示SF3B14与U2 snRNP的相互作用,证明其在RNA剪接中的关键调控作用。

2. **文献名称**:*Development and validation of a polyclonal antibody targeting human SF3B14 for cancer biomarker studies*

**作者**:Garcia R, Kim S, et al.

**摘要**:报道一种新型兔源多克隆抗体的开发,通过Western blot和免疫荧光验证其特异性,并应用于肺癌组织样本中SF3B14的异常表达分析。

3. **文献名称**:*SF3B14 depletion disrupts spliceosome assembly and induces genome instability*

**作者**:Müller P, Schmidt A, et al.

**摘要**:通过CRISPR敲低结合SF3B14抗体的免疫荧光定位,发现SF3B14缺失导致剪接体组装缺陷和DNA损伤反应激活。

4. **文献名称**:*Proteomic profiling identifies SF3B14 as a novel interactor of oncogenic splicing factors*

**作者**:Tanaka H, Ito T, et al.

**摘要**:采用SF3B14抗体进行质谱分析,鉴定其与多种癌相关剪接因子(如SRSF1)的相互作用,提示其在肿瘤发生中的潜在作用。

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**注意**:以上文献为模拟示例,实际研究中SF3B14可能以不同名称或复合体亚基形式出现(如与SF3B1相关)。建议通过PubMed或Google Scholar以关键词“SF3B14 antibody”、“spliceosome SF3B14”或“PHF5A”(注:PHF5A是SF3B14的别名之一)进一步检索,并核实目标蛋白的命名准确性。

背景信息

The SF3B14 antibody targets the SF3B14 protein, a critical component of the SF3B complex within the spliceosome, which mediates pre-mRNA splicing. SF3B14 (also known as PHF5A) is a 14 kDa subunit that stabilizes the SF3B complex structure and facilitates its interaction with other splicing factors. It plays a role in recognizing branch site sequences during spliceosome assembly, ensuring precise excision of introns. Antibodies against SF3B14 are widely used to study spliceosome dynamics, protein localization, and interactions in molecular biology research. They are essential tools for techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate splicing mechanisms in normal and diseased states. Dysregulation of SF3B components, including SF3B14. is linked to splicing errors implicated in cancers, myelodysplastic syndromes, and neurodegenerative disorders. Researchers also utilize these antibodies to explore mutations in SF3B1 (a core SF3B subunit) and their impact on SF3B14 stability or function. The antibody’s specificity and reliability make it valuable for elucidating the role of aberrant splicing in disease pathogenesis and potential therapeutic targeting.

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