| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | activator of RNA decay; ARD-1; ARD1; EC 3.1.4.-; NIPP1 |
| Entrez GeneID | 5511; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human PPP1R8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PPP1R8抗体的3篇参考文献及其摘要内容(基于公开信息模拟，非真实文献)：
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1. **文献名称**: "Characterization of PPP1R8/NIPP1 Antibody for Chromatin Localization Studies"
**作者**: van Driel R, et al.
**摘要**: 该研究开发了一种针对PPP1R8蛋白的高特异性多克隆抗体，通过免疫荧光和免疫沉淀技术验证其在HeLa细胞中的定位，发现PPP1R8主要富集于核内染色质活跃转录区域，提示其与RNA剪切复合物的潜在关联。
2. **文献名称**: "PPP1R8 Regulates Cell Cycle Progression via Phosphatase Interaction Networks"
**作者**: Boudrez A, et al.
**摘要**: 使用商业化PPP1R8单克隆抗体(克隆号3C2)进行Western blot和共聚焦显微镜分析，发现PPP1R8通过招募蛋白磷酸酶PP1γ调控G2/M期转换，敲低实验显示其缺失导致细胞周期阻滞及染色体分离异常。
3. **文献名称**: "Antibody Validation for PPP1R8 in Cancer Biomarker Screening"
**作者**: Li X, et al.
**摘要**: 研究系统性验证了多种PPP1R8抗体在乳腺癌组织中的特异性，发现部分抗体存在非特异性结合问题，最终推荐兔源单抗(货号ab12345)用于免疫组化，并证实PPP1R8在转移性癌组织中表达显著下调。
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注：上述文献为模拟示例，实际研究中建议通过PubMed或抗体供应商(如CST、Abcam)官网查询具体引用。
PPP1R8 (Protein Phosphatase 1 Regulatory Subunit 8), also known as NIPP1 (Nuclear Inhibitor of Protein Phosphatase 1), is a regulatory protein that interacts with protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in diverse cellular processes. PPP1R8 functions as a scaffold protein, modulating PP1 activity and substrate specificity, and plays critical roles in transcriptional regulation, RNA processing, chromatin remodeling, and cell cycle control. It is particularly implicated in maintaining genomic stability by participating in DNA damage repair and mitotic regulation. Dysregulation of PPP1R8 has been linked to cancer progression, neurodegenerative disorders, and developmental defects.
Antibodies targeting PPP1R8 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate PPP1R8's role in cellular signaling pathways, its phosphorylation status, and its association with PP1 or other binding partners. These antibodies also aid in exploring PPP1R8's dual functionality as both a tumor suppressor and promoter, depending on cellular context. Available as monoclonal or polyclonal variants, PPP1R8 antibodies are typically validated for specificity across human, mouse, and rat models, ensuring reliability in translational research. Their application spans cancer biology, neurobiology, and studies of epigenetic regulation.
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