| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NELL2 |
| Uniprot No | Q99435 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-816aa |
| 氨基酸序列 | LGVDPSLQI DVLTELELGE STTGVRQVPG LHNGTKAFLF QDTPRSIKAS TATAEQFFQK LRNKHEFTIL VTLKQTHLNS GVILSIHHLD HRYLELESSG HRNEVRLHYR SGSHRPHTEV FPYILADDKW HKLSLAISAS HLILHIDCNK IYERVVEKPS TDLPLGTTFW LGQRNNAHGY FKGIMQDVQL LVMPQGFIAQ CPDLNRTCPT CNDFHGLVQK IMELQDILAK TSAKLSRAEQ RMNRLDQCYC ERTCTMKGTT YREFESWIDG CKNCTCLNGT IQCETLICPN PDCPLKSALA YVDGKCCKEC KSICQFQGRT YFEGERNTVY SSSGVCVLYE CKDQTMKLVE SSGCPALDCP ESHQITLSHS CCKVCKGYDF CSERHNCMEN SICRNLNDRA VCSCRDGFRA LREDNAYCED IDECAEGRHY CRENTMCVNT PGSFMCICKT GYIRIDDYSC TEHDECITNQ HNCDENALCF NTVGGHNCVC KPGYTGNGTT CKAFCKDGCR NGGACIAANV CACPQGFTGP SCETDIDECS DGFVQCDSRA NCINLPGWYH CECRDGYHDN GMFSPSGESC EDIDECGTGR HSCANDTICF NLDGGYDCRC PHGKNCTGDC IHDGKVKHNG QIWVLENDRC SVCSCQNGFV MCRRMVCDCE NPTVDLFCCP ECDPRLSSQC LHQNGETLYN SGDTWVQNCQ QCRCLQGEVD CWPLPCPDVE CEFSILPENE CCPRCVTDPC QADTIRNDIT KTCLDEMNVV RFTGSSWIKH GTECTLCQCK NGHICCSVDP QCLQEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NELL2重组蛋白的参考文献示例(内容基于模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**：*NELL2 promotes neuronal survival through activation of the PI3K/Akt signaling pathway*
**作者**：Kim, J., et al.
**摘要**：研究通过重组NELL2蛋白处理神经元细胞，发现其能激活PI3K/Akt通路，显著抑制凋亡并促进轴突生长，提示其在神经退行性疾病治疗中的潜力。
2. **文献名称**：*Structural characterization of recombinant NELL2 and its role in craniofacial development*
**作者**：Zhang, Y., et al.
**摘要**：利用大肠杆菌系统表达并纯化NELL2重组蛋白，解析其结构特征，并通过小鼠模型证实NELL2缺失导致颅面发育异常，强调其在胚胎发育中的关键作用。
3. **文献名称**：*NELL2重组蛋白抑制乳腺癌细胞迁移的机制研究*
**作者**：Wang, L., et al.
**摘要**：体外实验表明，NELL2重组蛋白通过下调MMP-9表达抑制乳腺癌细胞侵袭转移，为癌症靶向治疗提供了新思路。
4. **文献名称**：*Recombinant NELL2 enhances synaptic plasticity in a rodent model of Alzheimer's disease*
**作者**：Smith, R., et al.
**摘要**：在阿尔茨海默病模型中，脑内注射NELL2重组蛋白改善了突触可塑性和记忆功能，机制涉及β-catenin信号通路的调控。
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**注意**：以上为模拟示例，实际文献需通过**PubMed/Google Scholar**检索关键词(如"NELL2 recombinant protein")获取。建议优先选择近5年发表、高被引或权威期刊论文以确保准确性。
NELL2 (Neural Epidermal Growth Factor-Like Like 2) is a secreted glycoprotein belonging to the NELL family, initially identified for its neural tissue expression and structural resemblance to epidermal growth factor (EGF)-like domains. It plays regulatory roles in cell survival, differentiation, and tissue development, particularly within the nervous system. NELL2 contains multiple functional domains, including thrombospondin type-1 repeats and epidermal growth factor-like repeats, enabling interactions with extracellular matrix components and cell surface receptors like integrins or receptor tyrosine kinases.
Recombinant NELL2 protein is engineered through genetic modification, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications. Its production allows researchers to study mechanistic pathways in vitro and in vivo. Studies suggest NELL2 participates in axon guidance, synaptic plasticity, and neuroprotection, with implications in neurodegenerative diseases (e.g., Alzheimer’s) or neural injury repair. It also exhibits tumor-suppressive properties in certain cancers by modulating cell proliferation and apoptosis pathways.
Recent research highlights NELL2’s involvement in non-neural contexts, such as bone metabolism, angiogenesis, and immune regulation. Its interaction with kinases like PROK2 or Wnt signaling components further broadens its functional scope. Despite progress, NELL2’s full receptor network and tissue-specific roles remain under investigation. Recombinant NELL2 serves as a critical tool for dissecting these pathways and exploring therapeutic applications in regenerative medicine or targeted cancer therapies. Ongoing studies aim to clarify its dual roles in health and disease, emphasizing its potential as a biomarker or biologic drug candidate.
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