| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUV420H2 |
| Uniprot No | Q86Y97 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 氨基酸序列 | MGPDRVTARE LCENDDLATS LVLDPYLGFR THKMNVSPVP PLRRQQHLRS ALETFLRQRD LEAAYRALTL GGWTARYFQS RGPRQEAALK THVYRYLRAF LPESGFTILP CTRYSMETNG AKIVSTRAWK KNEKLELLVG CIAELREADE GLLRAGENDF SIMYSTRKRS AQLWLGPAAF INHDCKPNCK FVPADGNAAC VKVLRDIEPG DEVTCFYGEG FFGEKNEHCE CHTCERKGEG AFRTRPREPA LPPRPLDKYQ LRETKRRLQQ GLDSGSRQGL LGPRACVHPS PLRRDPFCAA CQPLRLPACS ARPDTSPLWL QWLPQPQPRV RPRKRRRPRP RRAPVLSTHH AARVSLHRWG GCGPHCRLRG EALVALGQPP HARWAPQQDW HWARRYGLPY VVRVDLRRLA PAPPATPAPA GTPGPILIPK QALAFAPFSP PKRLRLVVSH GSIDLDVGGE EL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SUV420H2重组蛋白的模拟参考文献示例(非真实文献，仅作参考)：
1. **文献名称**：*SUV420H2-Mediated H4K20 Trimethylation Regulates Genomic Stability*
**作者**：Chen L, et al.
**摘要**：研究揭示了SUV420H2重组蛋白通过催化组蛋白H4K20的三甲基化(H4K20me3)，在DNA损伤修复和基因组稳定性维持中的关键作用，并探讨了其与癌症发生的关系。
2. **文献名称**：*Structural Insights into the Catalytic Mechanism of SUV420H2*
**作者**：Park S, et al.
**摘要**：通过X射线晶体学解析了SUV420H2重组蛋白的催化结构域，阐明了其底物识别和甲基转移活性的分子机制，为靶向该酶的药物设计提供了结构基础。
3. **文献名称**：*Expression and Purification of Recombinant SUV420H2 for Epigenetic Studies*
**作者**：Zhang Y, et al.
**摘要**：报道了一种高效表达和纯化重组SUV420H2蛋白的方法，验证了其体外甲基转移酶活性，并应用于筛选小分子抑制剂。
4. **文献名称**：*SUV420H2 Interaction with HP1 Proteins in Heterochromatin Formation*
**作者**：Müller R, et al.
**摘要**：研究发现SUV420H2重组蛋白与异染色质蛋白HP1的相互作用，揭示了其在异染色质组装和基因沉默中的协同调控机制。
(注：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索。)
**Background of SUV420H2 Recombinant Protein**
SUV420H2 (Suppressor of Variegation 4-20 Homolog 2) is a histone methyltransferase belonging to the Suv4-20 family, which plays a critical role in epigenetic regulation. It catalyzes the trimethylation of histone H4 at lysine 20 (H4K20me3), a post-translational modification associated with heterochromatin formation, genome stability, and transcriptional silencing. This enzymatic activity is essential for proper chromatin compaction during cell cycle progression, DNA repair, and telomere maintenance.
Structurally, SUV420H2 contains a conserved SET (Su(var)3-9. Enhancer-of-zeste, Trithorax) domain responsible for its methyltransferase activity. It typically functions in coordination with SUV420H1. another member of the Suv4-20 family, which initiates H4K20 monomethylation (H4K20me1). SUV420H2 then processes H4K20me1 to H4K20me3. highlighting its role in the stepwise methylation process. Dysregulation of SUV420H2 has been linked to genomic instability, developmental disorders, and cancers, underscoring its importance in maintaining cellular homeostasis.
Recombinant SUV420H2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Purified recombinant variants, often fused with tags like GST or His, retain enzymatic activity and are widely used to investigate H4K20me3 dynamics *in vitro*. Researchers employ this protein to explore its substrate specificity, interaction partners, and inhibition mechanisms, aiding drug discovery efforts targeting epigenetic disorders. Additionally, it serves as a tool to study chromatin remodeling pathways and their implications in diseases, making SUV420H2 a focal point in epigenetics and cancer research.
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