| 纯度 | >85%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Klrb1a |
| Uniprot No | P27811 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MDTARVYFGLKPPRTPGAWHESPPSLPPDACRCPRSHRLALKLSCAGLILLVVTLIGMSVLVRVLIQKPSIEKCYVLIQENLNKTTDCSAKLECPQDWLSHRDKCFHVSHVSNTWEEGLVDCDGKGATLMLIQDQEELRFLLDSIKEKYNSFWIGLRYTLPDMNWKWINGSTLNSDVLKITDDTENDSCAAISGDKVTFESCNSDNRWICQKELYHETLSNYVGYGH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Klrb1a重组蛋白的3篇参考文献示例(内容基于模拟生成，非真实文献)：
1. **文献名称**：*Structural characterization of the mouse Klrb1a receptor and its ligand interaction*
**作者**：Smith A, et al.
**摘要**：本研究通过重组表达Klrb1a蛋白并解析其晶体结构，揭示了其与配体Clr-b结合的分子机制，阐明了NK细胞活化中的关键构象变化。
2. **文献名称**：*Klrb1a recombinant protein modulates T cell response in autoimmune disease models*
**作者**：Zhang L, et al.
**摘要**：利用重组Klrb1a蛋白阻断NKR-P1A信号通路，发现其可抑制小鼠实验性自身免疫性脑脊髓炎(EAE)的炎症反应，提示其在自身免疫病治疗中的潜在应用价值。
3. **文献名称**：*Expression and functional analysis of recombinant Klrb1a in NK cell-mediated cytotoxicity*
**作者**：Tanaka K, et al.
**摘要**：通过哺乳动物细胞系统表达Klrb1a重组蛋白，证实其参与NK细胞对肿瘤细胞的杀伤作用，并发现特定糖基化修饰影响其受体功能。
(注：以上文献为示例性质，实际引用请以真实数据库检索结果为准。)
Klrb1a (Killer cell lectin-like receptor subfamily B member 1A) is a gene encoding a cell surface receptor protein primarily expressed on natural killer (NK) cells and subsets of T lymphocytes. This protein, also known as CD161 or NKR-P1A in humans, belongs to the C-type lectin-like receptor family and plays a critical role in modulating immune responses. It functions as an activating receptor involved in the regulation of cytotoxicity, cytokine production, and cell-cell interactions within the innate and adaptive immune systems. Klrb1a recognizes specific ligands, such as lectin-like transcript 1 (LLT1/CLEC2D), to mediate signaling pathways that balance immune activation and inhibition.
Recombinant Klrb1a protein is engineered through molecular cloning techniques, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications and structural integrity. The purified protein retains functional domains essential for ligand binding and signal transduction, making it a valuable tool for studying receptor-ligand interactions, immune cell activation mechanisms, and downstream signaling events. Researchers utilize Klrb1a recombinant proteins in flow cytometry, ELISA, and functional assays to investigate its role in infectious diseases, cancer immunosurveillance, and autoimmune disorders. Its involvement in NK cell-mediated cytotoxicity also positions it as a potential target for immunotherapeutic strategies aimed at enhancing antitumor responses or controlling chronic inflammation. Studies on Klrb1a contribute to a deeper understanding of immune checkpoint regulation and the development of biologics targeting C-type lectin pathways.
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