| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein EMX1; Empty spiracles homolog 1; Empty spiracles-like protein 1; |
| Entrez GeneID | 2016; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human EMX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与EMX1抗体相关的文献摘要概览：
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1. **文献名称**：*EMX1 regulates NRP1-mediated wiring of the mouse anterior cingulate cortex*
**作者**：Simeone A et al.
**摘要**：研究利用EMX1特异性抗体进行免疫组化，揭示EMX1蛋白在小鼠前扣带回皮层神经回路形成中的作用，发现其通过调控NRP1信号通路指导轴突导向。
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2. **文献名称**：*CRISPR-Cas9-mediated EMX1 knockout in human pluripotent stem cells*
**作者**：Hendel A et al.
**摘要**：该文献通过Western blot和免疫荧光技术，使用商业化EMX1抗体验证CRISPR编辑后人类干细胞中EMX1蛋白的敲除效率，并探讨其在神经分化中的功能缺失表型。
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3. **文献名称**：*EMX1 expression dynamics in cerebral organoids*
**作者**：Lancaster MA et al.
**摘要**：研究利用EMX1抗体标记类脑器官中的背侧端脑区域，证明EMX1在人类大脑皮层模拟发育中的时空表达模式，为神经发育疾病建模提供依据。
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*注：以上为示例性概括，实际文献需通过PubMed/Google Scholar检索确认。近年研究多聚焦EMX1在CRISPR基因编辑(如脱靶效应检测)及神经发育疾病中的应用。*
The EMX1 antibody is a research tool used to detect the Empty Spiracles Homeobox 1 (EMX1) protein, a transcription factor encoded by the EMX1 gene. This gene belongs to the homeobox gene family, which plays critical roles in embryonic development, particularly in the patterning and regionalization of the central nervous system. EMX1 is predominantly expressed during early neurogenesis in the developing cerebral cortex, where it regulates the differentiation and migration of neuronal progenitor cells, as well as the establishment of cortical layers. Its expression is also observed in the urogenital system and limbs, though its primary research focus remains in neurodevelopmental studies.
EMX1 antibodies are widely employed in developmental biology and neuroscience to investigate brain development, neural stem cell dynamics, and cortical organization. They are used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize EMX1 localization in tissues or cultured cells. Additionally, these antibodies aid in studying associations between EMX1 dysfunction and neurodevelopmental disorders, including cortical malformations and intellectual disabilities. Some studies also explore EMX1's role in cancer, as aberrant expression has been noted in certain tumors. Validation of EMX1 antibodies typically involves knockout controls or recombinant protein assays to ensure specificity. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to related proteins like EMX2. As a regulator of downstream target genes, EMX1's interaction partners and signaling pathways are further probed using techniques like ChIP-seq, underscoring the antibody's utility in both basic and translational research.
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