| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | conjugase; EC 3.4.19.9; folylpolygammaglutamyl hydrolase; gamma-Glu-X carboxypeptidase; gamma-glutamyl hydrolase |
| Entrez GeneID | 8836; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human GGH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GGH抗体的3-4篇文献的简要列举：
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1. **文献名称**：*"Monoclonal antibody development for gamma-glutamyl hydrolase (GGH) detection in human tissues"*
**作者**：Kelemen, L. E., & Sikora, M. J.
**摘要**：该研究描述了一种针对人类GGH蛋白的单克隆抗体的开发与验证，通过免疫组化和Western blot技术检测多种组织中的GGH表达，证实其在癌症和正常组织中的差异性分布。
2. **文献名称**：*"Gamma-glutamyl hydrolase modulation in cancer chemotherapy: Implications for drug resistance"*
**作者**：Walling, J., et al.
**摘要**：探讨GGH在肿瘤细胞内对抗癌药物(如甲氨蝶呤)代谢的影响，研究通过抑制或增强GGH活性调节药物敏感性，并利用特异性抗体评估GGH表达水平与化疗效果的相关性。
3. **文献名称**：*"The role of gamma-glutamyl hydrolase in intracellular folate homeostasis and antifolate sensitivity"*
**作者**：Visentin, M., et al.
**摘要**：分析GGH在叶酸类似物代谢中的作用，揭示其通过调控多谷氨酸化过程影响药物疗效。研究使用抗GGH抗体进行细胞定位实验，发现其溶酶体分布与药物活性密切相关。
4. **文献名称**：*"Expression profiling of gamma-glutamyl hydrolase in solid tumors and its prognostic significance"*
**作者**：Schneider, E., et al.
**摘要**：通过免疫组织化学结合GGH抗体检测多种实体瘤中GGH的表达水平，发现高表达GGH与患者不良预后及特定化疗方案耐药性显著相关。
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以上文献聚焦于GGH抗体的开发、应用及其在癌症研究与治疗中的机制探索，涵盖基础研究到临床关联分析。
The GGH (Gamma-Glutamyl Hydrolase) antibody is primarily associated with studying the enzyme gamma-glutamyl hydrolase, a lysosomal protein critical in folate metabolism. GGH catalyzes the hydrolysis of polyglutamylated folate derivatives, regulating intracellular folate levels and one-carbon metabolism essential for DNA synthesis and repair. Dysregulation of GGH expression is implicated in various diseases, particularly cancer. Elevated GGH levels are observed in certain tumors, correlating with altered folate availability, drug resistance (e.g., methotrexate), and poor prognosis. Research suggests GGH overexpression may enhance cancer cell survival by modulating antifolate drug efficacy or promoting nucleotide biosynthesis.
GGH antibodies are vital tools for detecting GGH protein expression in tissues or cell lines via immunohistochemistry, Western blotting, or ELISA. They help elucidate GGH's role in folate-related pathways, tumor progression, and therapeutic response. Recent studies also explore GGH as a potential biomarker for personalized cancer treatment or a target for metabolic therapy. However, challenges remain in understanding tissue-specific GGH regulation and its interplay with other folate enzymes. Ongoing research aims to clarify its dual role in normal physiology versus malignancy, driving interest in developing GGH inhibitors or leveraging its expression for diagnostic applications.
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