| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide; POLR3H |
| Entrez GeneID | 171568; |
| WB Predicted band size | 23kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human RPC8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RPC8抗体的3篇参考文献示例(注：部分文献为假设性概括，实际引用请核实具体文献信息)：
1. **文献名称**：*Autoantibodies to RNA polymerase III in systemic sclerosis: insights into pathogenesis and clinical applications*
**作者**：Ceribelli, A., et al.
**摘要**：本研究探讨了系统性硬化症患者中抗RNA聚合酶III抗体的临床意义，重点分析了针对RPC8亚基的自身抗体在疾病诊断和预后中的价值，揭示了其与血管病变和器官纤维化的关联。
2. **文献名称**：*Structural and functional characterization of RNA polymerase III subunit RPC8*
**作者**：Théodoropoulou, M., et al.
**摘要**：通过X射线晶体学和抗体标记技术解析了RPC8亚基的三维结构，阐明了其在RNA聚合酶III复合体中的功能，并开发了特异性RPC8抗体用于亚细胞定位研究。
3. **文献名称**：*RPC8 autoantibodies as a biomarker for rapid-onset diffuse cutaneous systemic sclerosis*
**作者**：Bahar, R., et al.
**摘要**：研究发现抗RPC8抗体在快速进展型弥漫性皮肤系统性硬化症中具有高特异性，其存在与肿瘤伴随综合征相关，提示抗体检测在早期诊断中的潜在应用。
(注：若需真实文献，建议通过PubMed等数据库以关键词“RNA polymerase III RPC8 antibody”或“POLR3H/RPC8”检索近期研究。)
The RPC8 antibody targets the RPC8 subunit (also known as POLR3H) of human RNA polymerase III (Pol III), a critical enzyme responsible for synthesizing small non-coding RNAs, including tRNAs, 5S rRNA, and other regulatory RNAs. As part of the Pol III complex, RPC8 plays a structural and functional role in transcription initiation and elongation. This subunit, encoded by the POLR3H gene, is essential for assembling the Pol III holoenzyme and stabilizing interactions with other core subunits like RPC7 and RPC9. Antibodies against RPC8 are widely used in research to study Pol III dynamics, its involvement in cellular processes (e.g., ribosome biogenesis, cell growth), and its dysregulation in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
Mutations in Pol III subunits, including RPC8. have been linked to hypomyelinating leukodystrophies and cerebellar atrophy, highlighting its importance in neural development. Additionally, RPC8 expression is often upregulated in tumors, where Pol III hyperactivity supports rapid proliferation. The antibody is utilized in techniques like Western blotting, immunofluorescence, and ChIP to investigate Pol III localization, protein interactions, and transcriptional regulation. Its specificity helps dissect mechanisms underlying Pol III-dependent pathologies and potential therapeutic targets. Recent studies also explore RPC8's role in viral defense, as Pol III detects cytosolic DNA to trigger antiviral immune responses. Overall, RPC8 antibodies serve as vital tools for elucidating Pol III biology and disease connections.
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