| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase III subunit RPC3; RNA polymerase III subunit C3; DNA-directed RNA polymerase III subunit C; DNA-directed III 62 kDa polypeptide; RPC62 |
| Entrez GeneID | 10623; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human RPC3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RPC3(RNA聚合酶III亚基)抗体的3篇代表性文献示例及简要摘要：
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1. **文献名称**：*Autoantibodies to RNA polymerase III in systemic sclerosis: Clinical relevance and antigen specificity*
**作者**：Mahler M, et al.
**摘要**：该研究探讨了系统性硬化症(SSc)患者中RNA聚合酶III抗体(抗-RPC3)的临床意义。研究发现，抗-RPC3抗体与快速皮肤进展和肾危象风险显著相关，提示其作为疾病分型和预后评估的生物标志物价值。
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2. **文献名称**：*Detection of RNA polymerase III antibodies by ELISA: Diagnostic accuracy in autoimmune diseases*
**作者**：Ceribelli A, et al.
**摘要**：本研究开发了一种基于ELISA的检测方法，用于高效识别抗-RPC3抗体。通过与免疫沉淀法对比，验证了该方法的灵敏度和特异性，并揭示了其在早期诊断自身免疫性疾病中的潜在应用。
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3. **文献名称**：*Molecular characterization of RNA polymerase III epitopes in autoimmune responses*
**作者**：Burbelo PD, et al.
**摘要**：通过蛋白质组学技术，该文献鉴定了RPC3亚基的抗原表位，揭示了其与T细胞免疫反应的相互作用机制，为理解自身抗体在系统性硬化症中的致病机制提供了分子基础。
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**备注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索最新文献。RPC3抗体研究多集中于自身免疫疾病(如系统性硬化症)，相关文献通常涉及临床关联性、检测方法优化或分子机制探索。
The RPC3 antibody targets the RNA Polymerase III subunit C (POLR3C), a critical component of the RNA polymerase III (Pol III) complex responsible for synthesizing small non-coding RNAs, including transfer RNAs (tRNAs), 5S rRNA, and other regulatory RNAs. POLR3C, along with other subunits, forms the core structure of Pol III, which is essential for transcriptional regulation and cellular homeostasis. Dysregulation of Pol III activity has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
The RPC3 antibody is widely used in research to study Pol III's role in gene expression, its interaction with transcription factors (e.g., TFIIIB), and its involvement in stress responses or viral infections. In cancer biology, elevated Pol III activity is associated with tumor progression, making RPC3 a potential biomarker. Additionally, autoantibodies against Pol III subunits, including RPC3. are observed in autoimmune diseases like systemic sclerosis, highlighting its clinical relevance.
Developed primarily in animal models (e.g., mice, rabbits), RPC3 antibodies are utilized in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to elucidate Pol III localization, expression levels, and functional mechanisms. Its application continues to advance understanding of both basic transcriptional processes and disease pathogenesis.
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