| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; FLJ10816; FLJ21921 |
| Entrez GeneID | 84172; |
| WB Predicted band size | 129kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from C-terminal of human POLR1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLR1B抗体的3篇参考文献示例(虚构示例，仅作格式参考)：
1. **标题**：POLR1B Antibody Characterization in Ribosome Biogenesis Studies
**作者**：Smith J, et al.
**摘要**：该研究验证了一种高特异性POLR1B多克隆抗体的应用，证实其在人细胞核仁定位分析中的有效性，揭示了POLR1B在rRNA转录中的动态调控。
2. **标题**：Role of POLR1B in Craniofacial Development: Insights from Antibody-Based Knockdown
**作者**：Lee H, et al.
**摘要**：通过POLR1B抗体介导的功能抑制实验，发现POLR1B缺失导致斑马鱼胚胎颅面发育异常，提示其在RNA聚合酶I组装中的关键作用。
3. **标题**：POLR1B as a Biomarker in Colorectal Cancer: Immunohistochemical Analysis
**作者**：Garcia R, et al.
**摘要**：利用POLR1B单克隆抗体进行组织染色，发现POLR1B在结直肠癌中高表达，且与患者预后不良相关，可能成为潜在治疗靶点。
注：以上为模拟内容，实际文献需通过PubMed或Google Scholar等平台检索关键词“POLR1B antibody”获取。
POLR1B (RNA Polymerase I Subunit B) is a core component of RNA polymerase I, a critical enzyme responsible for synthesizing ribosomal RNA (rRNA) in the nucleolus. As part of the Pol I complex, POLR1B plays a central role in transcription of the 45S rRNA precursor, a fundamental step in ribosome biogenesis, which is tightly linked to cell growth and proliferation. Dysregulation of POLR1B has been implicated in diseases such as ribosomopathies, developmental disorders, and cancers, where altered rRNA synthesis may drive pathological cell proliferation or apoptosis.
POLR1B antibodies are essential tools for studying the expression, localization, and function of this protein in both physiological and disease contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess POLR1B levels in cellular or tissue samples, visualize its nucleolar distribution, or explore interactions within the Pol I complex. Specific POLR1B antibodies help elucidate mechanisms underlying ribosomal stress responses, cancer progression, or genetic disorders like Treacher Collins syndrome, which involves mutations in RNA polymerase subunits. Validated antibodies are crucial for ensuring specificity, particularly given the structural similarities among RNA polymerase subunits. Researchers often verify POLR1B antibody performance using knockout cell lines or siRNA-mediated knockdown to confirm target selectivity.
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