| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5' nucleotidase; cytosolic IB; 5NT1B; AIRP; EC 3.1.3.5 |
| Entrez GeneID | 100526794;93034; |
| WB Predicted band size | 69kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human NT5C1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NT5C1B抗体的3篇参考文献示例(注：文献为虚构示例，实际文献需根据具体数据库检索)：
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1. **文献名称**: *"NT5C1B mutations drive chemoresistance in acute lymphoblastic leukemia through altered nucleotide metabolism"*
**作者**: Claire, J. et al.
**摘要**: 研究报道了NT5C1B在急性淋巴细胞白血病(ALL)中的功能突变，发现其通过调控核苷酸代谢通路导致化疗药物耐药性。研究利用特异性NT5C1B抗体进行蛋白表达分析，证实突变体与不良预后相关。
2. **文献名称**: *"Development of a monoclonal antibody targeting NT5C1B for diagnostic applications in solid tumors"*
**作者**: Smith, R. & Patel, A.
**摘要**: 本研究开发了一种高特异性抗NT5C1B单克隆抗体，验证其在结直肠癌和肺癌组织中的诊断潜力。抗体通过免疫组化证实NT5C1B在肿瘤细胞中高表达，并与转移风险相关。
3. **文献名称**: *"NT5C1B as a novel biomarker for immune checkpoint therapy response in melanoma"*
**作者**: Zhang, L. et al.
**摘要**: 通过流式细胞术和Western blot(使用NT5C1B抗体)，研究发现NT5C1B表达水平与黑色素瘤患者对PD-1抑制剂的治疗响应呈负相关，提示其可作为免疫治疗耐药性的预测标志物。
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如需真实文献，建议通过PubMed、Google Scholar等平台以“NT5C1B antibody”、“NT5C1B function”或“NT5C1B biomarker”为关键词检索。
The NT5C1B antibody targets the 5'-nucleotidase cytosolic IB protein, encoded by the NT5C1B gene. This enzyme belongs to the 5'-nucleotidase family, which catalyzes the dephosphorylation of nucleoside monophosphates (e.g., AMP, GMP) to generate nucleosides, regulating intracellular nucleotide pools and salvage pathways. NT5C1B is implicated in purine metabolism, cellular signaling, and energy homeostasis. Dysregulation of NT5C1B has been associated with metabolic disorders, immune dysfunction, and cancer, particularly due to its role in nucleotide recycling and potential impact on cell proliferation.
Antibodies against NT5C1B are widely used in research to study its expression, localization, and function in tissues or cell lines. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the exploration of NT5C1B's involvement in diseases such as leukemia, where mutations in related nucleotidases have been linked to drug resistance. These antibodies are often validated for specificity through knockout controls or siRNA silencing.
Current studies focus on NT5C1B's interaction with metabolic pathways and its potential as a therapeutic target. Species reactivity typically includes human, mouse, and rat, supporting cross-species translational research. Understanding NT5C1B's mechanisms may uncover novel strategies for treating metabolic syndromes or cancers driven by nucleotide imbalance.
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