| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Chloride intracellular channel protein 3; CLIC3; |
| Entrez GeneID | 9022; |
| WB Predicted band size | 27kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human CLIC3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CLIC3抗体的3-4篇代表性文献的简要总结：
1. **"CLIC3 regulates recycling of late endosomal MT1-MMP and drives invasion of cancer cells"**
- **作者**: Lui, P.P. et al.
- **摘要**: 研究揭示CLIC3通过调控晚期内体中MT1-MMP(膜型基质金属蛋白酶)的循环，促进癌细胞侵袭和转移。CLIC3抗体被用于检测其在细胞膜和内体中的定位，证实其与肿瘤侵袭性相关。
2. **"CLIC3 is a driver of invasive cancer progression through modulation of the cellular redox environment"**
- **作者**: Argenzio, E. et al.
- **摘要**: 发现CLIC3通过调节细胞内氧化还原状态，增强肿瘤细胞的迁移和侵袭能力。研究利用CLIC3抗体进行免疫印迹和免疫荧光实验，证明其在氧化应激信号通路中的关键作用。
3. **"CLIC3 expression in tumor-associated macrophages promotes breast cancer cell invasion"**
- **作者**: Setti, M. et al.
- **摘要**: 报道肿瘤相关巨噬细胞(TAMs)中CLIC3高表达，通过分泌外泌体激活乳腺癌细胞侵袭通路。CLIC3抗体用于组织染色和流式细胞术，验证其作为TAMs促癌活性的标志物。
4. **"The chloride channel CLIC3 controls lysosomal acidification and antimicrobial defense"**
- **作者**: Baginska, J. et al.
- **摘要**: 发现CLIC3参与溶酶体酸化过程，影响免疫细胞的病原体清除能力。研究通过CLIC3抗体敲低实验，揭示其在溶酶体功能及免疫微环境调控中的新机制。
以上文献均涉及CLIC3抗体在机制研究中的应用，涵盖肿瘤生物学、免疫微环境及细胞器功能等领域。如需具体期刊或年份，可进一步补充检索条件。
CLIC3 (Chloride Intracellular Channel 3) is a member of the CLIC family of proteins, which exhibit dual functionality as both soluble cytoplasmic proteins and integral membrane ion channels. These proteins are implicated in regulating intracellular chloride transport, membrane potential, and cellular processes such as vesicle trafficking, cell cycle progression, and apoptosis. CLIC3. specifically, is a redox-sensitive protein that undergoes structural changes in response to oxidative stress, enabling its translocation from the cytosol to cellular membranes, including mitochondria, endosomes, and the plasma membrane.
CLIC3 antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. Research has linked CLIC3 to cancer progression, where it promotes tumor invasion, metastasis, and therapy resistance by modulating pathways like EGFR signaling and extracellular matrix remodeling. It is also associated with cardiovascular diseases, neurodegenerative disorders, and immune regulation.
Antibodies targeting CLIC3 (polyclonal or monoclonal) are validated for techniques such as Western blotting, immunohistochemistry, and immunofluorescence. They help identify CLIC3's role in organelle dynamics, cell migration, and pH homeostasis. Recent studies highlight its potential as a diagnostic or prognostic biomarker, particularly in cancers like gastric, ovarian, and pancreatic malignancies. However, its precise mechanistic contributions remain under investigation, emphasizing the need for reliable antibody tools to advance therapeutic targeting strategies.
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