| 纯度 | > 80 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CABP7 |
| Uniprot No | Q86V35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPFHPVTAALMYRGIYTVPNLLSEQRPVDI PEDELEEIREAFKVFDRDGNGFISKQELGTAMRSLGYMPNEVELEVIIQR LDMDGDGQVDFEEFVTLLGPKLSTSGIPEKFHGTDFDTVFWKCDMQKLTV DELKRLLYDTFCEHLSMKDIENIIMTEEESHLGTAEECPVDVETCSNQQI RQTCVRKS |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CABP7重组蛋白的3篇代表性文献(基于模拟生成内容，非真实文献)：
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1. **标题**：Expression and calcium-binding properties of recombinant CABP7 in neuronal cells
**作者**：Chen L, et al.
**摘要**：报道了人源CABP7重组蛋白在大肠杆菌中的高效表达与纯化，通过钙离子结合实验证实其与Ca²⁵的高亲和力，并揭示其在神经元突触传递中的潜在调控作用。
2. **标题**：Structural characterization of CABP7 and its interaction with voltage-gated calcium channels
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞系统表达重组CABP7.通过X射线晶体学解析其三维结构，发现其EF-hand结构域与L型钙通道的C末端存在特异性结合，提示其在钙稳态中的功能。
3. **标题**：CABP7 knockdown alters synaptic plasticity through recombinant protein rescue experiments
**作者**：Kimura S, et al.
**摘要**：构建了HEK293细胞表达的重组CABP7蛋白，通过神经元基因沉默及蛋白回补实验，证明CABP7通过调节钙依赖性激酶活性影响突触可塑性。
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注：以上为示例性内容，实际文献需通过PubMed/Google Scholar以“CABP7”或“calcium-binding protein 7 + recombinant”为关键词检索获取。
CABP7 (Calcium-binding protein 7) is a member of the neuronal calcium sensor (NCS) family, which plays critical roles in calcium-mediated signaling pathways. Initially identified through genomic analyses, CABP7 is expressed predominantly in the brain, particularly in regions like the cerebral cortex, hippocampus, and cerebellum, suggesting its involvement in neural functions. Structurally, it contains EF-hand motifs that enable calcium ion binding, a hallmark feature of proteins regulating intracellular calcium homeostasis. Unlike other NCS members like calmodulin or recoverin, CABP7 exhibits distinct expression patterns and functional specializations, potentially influencing synaptic plasticity, neurotransmitter release, or neuronal survival.
Recombinant CABP7 protein is engineered using expression systems like *E. coli* or mammalian cells to study its biochemical properties and interactions. Its production allows researchers to investigate calcium-dependent conformational changes, binding partners (e.g., membrane receptors or enzymes), and downstream signaling cascades. Studies suggest CABP7 may modulate calcium-sensitive processes in neurodegenerative diseases, such as Alzheimer's or Parkinson's, where disrupted calcium signaling contributes to pathology. Additionally, its role in cancer cell proliferation has been explored, as calcium signaling aberrations are common in malignancies.
The development of recombinant CABP7 also supports diagnostic or therapeutic applications, including antibody production for biomarker detection or screening small molecules targeting calcium signaling pathways. Despite progress, CABP7's precise physiological roles remain partially unresolved, driving ongoing research to clarify its contributions to cellular communication and disease mechanisms. This makes recombinant CABP7 a valuable tool for bridging molecular insights with translational biomedical applications.
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