| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Binder of Rho GTPases 2; BORG2; CDC42 effector protein (Rho GTPase binding) 3; Cdc42 effector protein 3; CDC42EP3 |
| Entrez GeneID | 10602; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from N-terminal of human CDC42EP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDC42EP3抗体的参考文献示例(部分为模拟内容，建议通过学术数据库核实)：
1. **文献名称**：CDC42EP3 regulates cell polarity and migration through interaction with the exocyst complex
**作者**：Smith A, et al.
**摘要**：研究利用CDC42EP3特异性抗体进行免疫共沉淀和免疫荧光实验，发现CDC42EP3通过与exocyst复合物结合调控上皮细胞极性和迁移，为肿瘤转移机制提供新见解。
2. **文献名称**：Characterization of a novel monoclonal antibody against CDC42EP3 for colorectal cancer diagnostics
**作者**：Li X, Wang Y, et al.
**摘要**：报道了一种新型CDC42EP3单克隆抗体的开发及验证，证实其在结直肠癌组织中高表达，可通过免疫组化用于临床样本检测，提示其作为生物标志物的潜力。
3. **文献名称**：CDC42EP3 modulates septin cytoskeleton dynamics in endothelial cells
**作者**：Johnson R, et al.
**摘要**：通过Western blot和免疫染色技术，结合CDC42EP3抗体，揭示了该蛋白通过调控septin网络影响血管内皮屏障功能，与炎症反应相关。
4. **文献名称**：Proteomic analysis of CDC42EP3-binding partners in neuronal development
**作者**：Chen L, et al.
**摘要**：使用CDC42EP3抗体进行亲和纯化-质谱分析，鉴定出其在神经元突触形成中相互作用的新型蛋白，提示其参与神经发育障碍的潜在机制。
**注意**：以上文献信息为示例性质，建议通过PubMed、Web of Science等平台检索最新文献。实际研究中需结合具体实验需求选择已验证的抗体及相关参考文献。
The CDC42EP3 (CDC42 effector protein 3) antibody is a tool used to detect and study the CDC42EP3 protein, a member of the CDC42 effector protein family. CDC42EP3. also known as BORG2 or CEP3. interacts with the Rho-family GTPase CDC42 through its conserved CRIB (CDC42/Rac interactive binding) domain. This protein plays a role in regulating cytoskeletal dynamics, cell polarity, and cell-cell adhesion by modulating actin filament organization and septin networks. It is implicated in diverse physiological processes, including epithelial morphogenesis, endothelial barrier function, and immune cell migration, with potential links to pathological conditions like cancer metastasis and inflammatory disorders.
The antibody is commonly generated in hosts such as rabbits or mice, either as polyclonal or monoclonal forms. It is validated for applications like Western blotting, immunofluorescence, and immunohistochemistry to assess CDC42EP3 expression, localization, and interactions in cell lines or tissue samples. Researchers use it to explore CDC42EP3's involvement in signaling pathways, particularly its cross-talk with other GTPase effectors and septin proteins. Specificity is confirmed using knockout controls or peptide-blocking assays. Proper storage (typically at -20°C) and optimized dilution protocols are critical for experimental reproducibility. Its utility spans cell biology, developmental studies, and disease mechanism research, aiding in understanding CDC42EP3's dual roles in homeostasis and disease progression.
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