| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | calcium-independent phospholipase A2-gamma; EC 3.1.1.5; intracellular membrane-associated calcium-independent phospholipase A2 gamma; iPLA2-gamma; patatin-like phospholipase domain-containing protein 8 |
| Entrez GeneID | 50640; |
| WB Predicted band size | 88kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human PNPLA8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PNPLA8抗体的3篇模拟文献摘要(注：文献为虚构示例，实际文献需通过数据库查询获取)：
1. **文献名称**：*PNPLA8 regulates mitochondrial membrane remodeling in neurodegeneration*
**作者**：Smith J, et al.
**摘要**：本研究利用特异性PNPLA8抗体，通过免疫印迹和免疫组化技术，揭示了PNPLA8在阿尔茨海默病模型小鼠脑组织线粒体中的高表达，并证实其通过水解磷脂参与线粒体膜动态调节，可能影响神经元存活。
2. **文献名称**：*Characterization of PNPLA8 in skeletal muscle regeneration*
**作者**：Johnson L, et al.
**摘要**：通过PNPLA8抗体介导的蛋白质定位分析，发现PNPLA8在受损肌肉细胞中显著上调，其磷脂酶活性可能通过调控细胞膜修复通路促进肌管形成，提示其在肌肉再生中的潜在作用。
3. **文献名称**：*PNPLA8 deficiency exacerbates hepatic steatosis via impaired lipid droplet hydrolysis*
**作者**：Lee S, et al.
**摘要**：利用PNPLA8基因敲除小鼠模型及抗体检测技术，证实PNPLA8缺失会导致肝脏脂滴水解能力下降，加重高脂饮食诱导的脂肪肝，表明其在脂代谢中的关键功能。
(如需真实文献，建议通过PubMed或Google Scholar以“PNPLA8 antibody”或“PNPLA8 function”为关键词检索。)
The PNPLA8 antibody is a tool used to study the PNPLA8 protein, a member of the patatin-like phospholipase domain-containing (PNPLA) family. PNPLA8. also known as calcium-independent phospholipase A2 gamma (iPLA2γ), is an enzyme encoded by the *PNPLA8* gene. It plays a critical role in phospholipid metabolism, particularly in the hydrolysis of glycerophospholipids to maintain membrane homeostasis and regulate lipid signaling. PNPLA8 is distinct from other phospholipases due to its calcium-independent enzymatic activity and localization to mitochondria, where it influences membrane remodeling, energy metabolism, and apoptosis.
Antibodies targeting PNPLA8 are essential for detecting its expression, localization, and functional roles in various tissues. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PNPLA8's involvement in physiological and pathological processes, including metabolic disorders, neurodegenerative diseases, and cancer. Research suggests PNPLA8's association with mitochondrial dysfunction, oxidative stress, and lipid peroxidation, highlighting its potential as a biomarker or therapeutic target. Validation of PNPLA8 antibodies typically includes specificity checks via knockout controls and enzymatic activity assays. These tools continue to advance understanding of lipid-mediated cellular mechanisms and disease pathways.
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