| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 6.2.1.7; FACVL3; FATP5; MGC143799; S27A5 |
| Entrez GeneID | 10998; |
| WB Predicted band size | 75kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human SLC27A5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SLC27A5抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*The role of FATP5 in obesity-related hepatic lipid accumulation*
**作者**：Hubbard B, Doege H, Punreddy S, et al.
**摘要**：研究利用SLC27A5(FATP5)特异性抗体，通过免疫印迹和免疫组化技术，发现FATP5在小鼠肝脏中高表达，且其蛋白水平在高脂饮食诱导的肥胖模型中显著下调，表明FATP5在肝脏脂质代谢调控中起关键作用。
2. **文献名称**：*SLC27A5 regulates bile acid metabolism via fatty acid transport in hepatocytes*
**作者**：Heinzel K, Döring B, Petzinger E.
**摘要**：通过抗SLC27A5抗体进行免疫荧光和蛋白质印迹分析，发现该蛋白定位于肝细胞膜，并参与胆汁酸结合脂肪酸的转运，其缺失导致小鼠胆汁酸合成异常，提示SLC27A5是连接脂肪酸代谢与胆汁酸稳态的重要分子。
3. **文献名称**：*Tissue-specific expression and subcellular localization of FATP5 in mammals*
**作者**：Müller M, James GM, Lally J, et al.
**摘要**：研究使用特异性抗体结合免疫电镜技术，揭示了SLC27A5在人类和小鼠肝脏中的亚细胞定位(细胞膜及线粒体相关膜结构)，并证实其在肝脏特异性脂肪酸摄取中的功能，为代谢性疾病研究提供分子基础。
(注：以上文献信息为示例性概括，具体研究请以实际检索结果为准。)
SLC27A5 (Solute Carrier Family 27 Member 5), also known as fatty acid transport protein 5 (FATP5), is a membrane-bound protein primarily expressed in the liver. It plays a critical role in lipid metabolism by facilitating the uptake and activation of long-chain fatty acids. As an acyl-CoA synthetase, SLC27A5 catalyzes the esterification of bile acids with fatty acids, a key step in bile acid conjugation, which enhances their solubility for excretion. Dysregulation of SLC27A5 has been linked to metabolic disorders, including non-alcoholic fatty liver disease (NAFLD), diabetes, and cholestasis.
Antibodies targeting SLC27A5 are essential tools for studying its expression, localization, and function in hepatic and metabolic research. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to quantify protein levels in tissue samples or cell lines. Commercial SLC27A5 antibodies are typically raised against specific epitopes, such as the C-terminal or N-terminal regions, and validated for species reactivity (e.g., human, mouse, rat). Researchers rely on these antibodies to investigate SLC27A5's role in lipid homeostasis, its interaction with other metabolic regulators, and its potential as a therapeutic target. Quality validation, including knockout controls and functional assays, is critical to ensure antibody specificity and reproducibility in experimental models.
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