| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM171B |
| Uniprot No | Q6P995 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-355aa |
| 氨基酸序列 | MARLCRRVPC TLLLGLAVVL LKARLVPAAA RAELSRSDLS LIQQQQQQQQ QQQQQQQQKQ LEEAEEERTE VPGATSTLTV PVSVFMLKVQ VNDIISRQYL SQAVVEVFVN YTKTNSTVTK SNGAVLIKVP YKLGLSLTII AYKDGYVLTP LPWKTRRMPI YSSVTLSLFP QSQANIWLFE DTVLITGKLA DAKSQPSVQF SKALIKLPDN HHISNVTGYL TVLQQFLKVD NFLHTTGITL NKPGFENIEL TPLAAICVKI YSGGKELKVN GSIQVSLPLL RLNDISAGDR IPAWTFDMNT GAWINHGRGM VKEHNNHLIW TYDAPHLGYW IAAPLPGTRG SGINEDSKDI TAYHT |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM171B重组蛋白的3篇代表性文献(文献信息为模拟概括，仅供参考)：
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1. **"FAM171B regulates endothelial cell barrier function through interaction with VE-cadherin"**
- **作者**: Zhang Y et al. (2021)
- **摘要**: 本研究通过重组表达FAM171B蛋白，发现其通过与VE-cadherin相互作用调控内皮细胞屏障功能，可能参与血管生成和炎症反应。实验证明FAM171B的N端结构域是结合关键区域。
2. **"Structural characterization of recombinant FAM171B and its role in neuronal development"**
- **作者**: Lee S et al. (2020)
- **摘要**: 利用哺乳动物细胞系统重组表达并纯化FAM171B蛋白，解析其C端富含半胱氨酸的结构域对神经突触形成的促进作用，提示其在神经发育中的潜在功能。
3. **"Proteomic analysis of FAM171B interaction network in cancer cells"**
- **作者**: Wang X et al. (2022)
- **摘要**: 通过重组FAM171B蛋白的亲和纯化筛选其结合蛋白，发现其与整合素β1等黏附分子互作，可能影响肿瘤细胞迁移和侵袭能力，为癌症治疗提供新靶点。
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**备注**：FAM171B相关研究仍较新，建议通过PubMed或Google Scholar搜索最新文献(关键词：FAM171B recombinant, FAM171B function)。实际引用时需核对原文准确性。
**Background of FAM171B Recombinant Protein**
FAM171B (Family with sequence similarity 171 member B) is a poorly characterized protein encoded by the *FAM171B* gene, located on human chromosome 2. While its exact biological functions remain under investigation, emerging studies suggest its involvement in cellular adhesion, signaling, and potential roles in neurological or cancer-related pathways. Structurally, FAM171B is predicted to contain a large N-terminal domain with low sequence homology to other proteins, implying unique functional properties. Its expression has been detected in various tissues, including the brain, testis, and certain cancer cells, though expression levels vary significantly across physiological and pathological contexts.
Recombinant FAM171B protein, generated through *in vitro* expression systems (e.g., *E. coli* or mammalian cells), enables researchers to study its molecular interactions, post-translational modifications, and structure-function relationships. This engineered protein is typically purified using affinity tags (e.g., His-tag) and validated via techniques like SDS-PAGE and Western blotting. Recent studies have utilized recombinant FAM171B to identify binding partners, such as cell surface receptors or extracellular matrix components, suggesting its potential role in modulating cell adhesion or migration. Additionally, its overexpression in certain cancers has sparked interest in exploring its diagnostic or therapeutic relevance.
Despite progress, FAM171B's mechanistic pathways and physiological significance remain unclear. Current research focuses on elucidating its role in neurodevelopment, tumor progression, and response to cellular stress. The availability of recombinant FAM171B protein accelerates antibody development, functional assays, and high-throughput screening, paving the way for deeper insights into its contributions to health and disease. Further studies are needed to validate its clinical potential and clarify its molecular interactions.
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