| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 4 ABC-type transport protein; ABC28; ATP-binding cassette sub-family F member 2; ATP-binding cassette; sub-family F (GCN20) |
| Entrez GeneID | 10061;114483834; |
| WB Predicted band size | 71kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ABCF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ABCF2抗体的模拟参考文献示例(仅供参考，非真实文献)：
1. **文献名称**：*ABCF2 overexpression correlates with poor prognosis in hepatocellular carcinoma and promotes tumor cell proliferation*
**作者**：Li X, Zhang Y, et al.
**摘要**：本研究通过免疫组织化学(使用ABCF2抗体)分析肝癌组织中ABCF2蛋白表达水平，发现其高表达与患者预后不良相关。体外实验表明ABCF2通过调控细胞周期蛋白加速肿瘤增殖。
2. **文献名称**：*ABCF2 modulates chemoresistance in breast cancer via regulating autophagy pathways*
**作者**：Smith JA, Brown KE, et al.
**摘要**：利用ABCF2抗体进行Western blot和免疫荧光实验，证实ABCF2在乳腺癌耐药细胞系中表达升高。研究揭示其通过激活自噬通路降低化疗药物敏感性，沉默ABCF2可逆转耐药性。
3. **文献名称**：*Subcellular localization and functional characterization of ABCF2 in lung adenocarcinoma*
**作者**：Tanaka H, Sato R, et al.
**摘要**：通过特异性ABCF2抗体结合共聚焦显微镜技术，发现ABCF2主要定位于线粒体膜。功能研究表明其通过调节线粒体膜电位影响肺癌细胞凋亡，提示其为潜在治疗靶点。
4. **文献名称**：*Development and validation of a novel monoclonal antibody against human ABCF2 for diagnostic applications*
**作者**：Wang L, Chen M, et al.
**摘要**：本文报道一种新型ABCF2单克隆抗体的开发与验证。该抗体在ELISA、免疫沉淀及组织芯片中表现高特异性，为ABCF2相关疾病的诊断和机制研究提供可靠工具。
(注：以上文献为模拟内容，实际引用需查询真实数据库。)
The ABCF2 antibody targets the ATP-binding cassette subfamily F member 2 (ABCF2), a protein belonging to the ATP-binding cassette (ABC) transporter superfamily. Unlike many ABC transporters involved in substrate translocation across membranes, ABCF2 lacks transmembrane domains and is primarily associated with intracellular processes. ABCF2 is implicated in ribosome biogenesis, translation regulation, and cellular stress responses. Studies suggest its role in modulating endoplasmic reticulum (ER) stress and unfolded protein response (UPR), potentially influencing cell survival under stress conditions.
ABCF2 is ubiquitously expressed, with elevated levels observed in tissues like the placenta, liver, and pancreas. Its dysregulation has been linked to cancers, including ovarian, lung, and hepatocellular carcinoma, where it may act as an oncogene or tumor suppressor depending on context. Research also explores its involvement in drug resistance, particularly in chemotherapy, highlighting its clinical relevance.
The ABCF2 antibody is a critical tool for detecting and quantifying ABCF2 protein expression in experimental settings. It enables applications such as Western blotting, immunohistochemistry, and immunofluorescence to study ABCF2's localization, expression patterns, and interactions. Researchers utilize this antibody to investigate ABCF2's mechanistic roles in cellular homeostasis, disease progression, and therapeutic targeting. Commercial antibodies are typically validated for specificity, often targeting conserved epitopes within the protein's nucleotide-binding domains. Continued interest in ABCF2 underscores its potential as a biomarker or therapeutic target in stress-related pathologies and oncology.
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