| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Angiomotin-like protein 1; |
| Entrez GeneID | 154810; |
| WB Predicted band size | 107kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human AMOTL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条与AMOTL1抗体相关的参考文献示例(内容基于公开研究概括，非真实文献)：
1. **文献名称**：*"AMOTL1 regulates endothelial cell polarity and vascular lumen formation"*
**作者**：Smith J, et al.
**摘要**：研究利用AMOTL1特异性抗体，通过免疫荧光和Western blot验证其在血管内皮细胞中的表达，揭示AMOTL1通过调控细胞极性蛋白促进血管腔形成。
2. **文献名称**：*"AMOTL1 interacts with YAP to modulate Hippo signaling in cancer"*
**作者**：Zhang L, et al.
**摘要**：通过免疫共沉淀(Co-IP)和抗体染色，证明AMOTL1与Hippo通路关键分子YAP结合，抑制其核定位，从而抑制肿瘤细胞增殖。
3. **文献名称**：*"AMOTL1 antibody-based profiling identifies its role in embryonic development"*
**作者**：Wang Y, et al.
**摘要**：使用AMOTL1抗体对小鼠胚胎组织进行免疫组化分析，发现其在神经管和心脏发育中高表达，提示其对胚胎形态发生至关重要。
4. **文献名称**：*"AMOTL1 as a biomarker in triple-negative breast cancer"*
**作者**：Kim H, et al.
**摘要**：通过AMOTL1抗体检测临床样本，发现其表达水平与乳腺癌患者预后显著相关，可能作为潜在治疗靶点。
(注：以上文献为示例性内容，实际引用时需以真实发表文献为准。)
**Background of AMOTL1 Antibody**
AMOTL1 (Angiomotin-like protein 1) is a member of the motin family, which includes proteins involved in regulating cell polarity, migration, and angiogenesis. It shares structural homology with angiomotin (AMOT), featuring conserved coiled-coil and PDZ-binding domains. AMOTL1 plays a role in the Hippo signaling pathway by interacting with YAP/TAZ transcription co-activators, influencing cell proliferation and organ size control. It also participates in maintaining endothelial cell junctions and modulating actin cytoskeleton dynamics.
The AMOTL1 antibody is a critical tool for detecting and studying the expression, localization, and function of AMOTL1 in various biological contexts. Researchers utilize this antibody in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore AMOTL1's involvement in developmental processes, cancer (e.g., tumor suppression or progression roles), and vascular diseases. Studies have linked AMOTL1 dysregulation to pathological conditions, including angiogenesis-dependent disorders and metastatic cancers, highlighting its therapeutic potential.
Available as monoclonal or polyclonal versions, AMOTL1 antibodies are typically raised in hosts like rabbits or mice, with validation in specific applications. Their use has advanced understanding of AMOTL1's interplay with signaling pathways and its role as a scaffold protein bridging membrane-associated proteins and cytoskeletal components.
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