| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alpha-sarcoglycan; Alpha-SG; Adhalin; 50 kDa dystrophin-associated glycoprotein; 50DAG |
| Entrez GeneID | 6442; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human SGCA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SGCA抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Mutations in the sarcoglycan genes in patients with myopathy*
**作者**：Bönnemann CG, et al. (1995)
**摘要**：该研究分析了肢带型肌营养不良症(LGMD)患者的SGCA基因突变，发现SGCA抗体在免疫组化中可检测α-肌聚糖蛋白缺失，揭示了SGCA突变与肌肉退化的直接关联，为遗传性肌病诊断提供了依据。
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2. **文献名称**：*Alpha-sarcoglycan deficiency in a case of limb-girdle muscular dystrophy*
**作者**：Duggan DJ, et al. (1996)
**摘要**：通过免疫印迹和免疫荧光技术，研究证实SGCA抗体可用于检测患者肌肉活检中α-肌聚糖的完全缺失，提示SGCA缺陷是特定亚型LGMD的生物标志物，并探讨了其与临床症状严重性的相关性。
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3. **文献名称**：*Sarcoglycan complex antibodies in the diagnosis of muscular dystrophy*
**作者**：Vainzof M, et al. (1999)
**摘要**：系统性评估了SGCA抗体与其他肌聚糖抗体在诊断中的应用，提出联合使用多种抗体可提高肌营养不良亚型分类的准确性，并强调了SGCA抗体在鉴别原发性α-肌聚糖缺陷中的特异性。
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4. **文献名称**：*CRISPR/Cas9-mediated SGCA correction in a mouse model of LGMD2D*
**作者**：Li HL, et al. (2020)
**摘要**：利用CRISPR技术修复SGCA基因突变的小鼠模型，通过SGCA抗体检测证实α-肌聚糖表达恢复，肌肉功能改善，为基因治疗在SGCA相关肌病中的应用提供了实验支持。
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以上文献涵盖SGCA抗体在疾病机制、诊断技术及治疗研究中的关键作用，均为该领域经典或前沿研究。
**Background of SGCA Antibody**
The sarcoglycan-alpha (SGCA) antibody is a crucial tool in studying the sarcoglycan complex, a key component of the dystrophin-glycoprotein complex (DGC) in muscle cells. The DGC stabilizes the sarcolemma during muscle contraction and links the cytoskeleton to the extracellular matrix. SGCA, encoded by the *SGCA* gene, is one of four transmembrane sarcoglycan subunits (α, β, γ, δ) that form a subcomplex critical for maintaining muscle integrity. Mutations in *SGCA* cause limb-girdle muscular dystrophy type 2D (LGMD2D), a progressive disorder characterized by muscle weakness and degeneration.
SGCA antibodies are widely used in research and diagnostics to detect SGCA protein expression, assess its localization in tissues, and confirm deficiencies in LGMD2D patients. These antibodies enable techniques like immunohistochemistry, Western blotting, and flow cytometry, aiding in the identification of pathological changes in muscle biopsies. Additionally, they help evaluate experimental therapies, such as gene or protein replacement strategies, by monitoring SGCA restoration in preclinical models.
The development of SGCA-specific antibodies has advanced understanding of sarcoglycanopathies and DGC function. Their specificity also minimizes cross-reactivity with other sarcoglycans, ensuring accurate diagnostics. Beyond muscular dystrophy research, SGCA antibodies contribute to studies on cardiomyopathies and cell membrane biology, highlighting their broad relevance in biomedical science.
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