| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | acetaldehyde dehydrogenase 5; AL1B1; aldehyde dehydrogenase X; mitochondrial; ALDH class 2 |
| Entrez GeneID | 219; |
| WB Predicted band size | 57kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ALDH1B1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ALDH1B1抗体的代表性文献概览：
1. **文献名称**: *ALDH1B1 is a potential stem/progenitor marker in human pancreas*
**作者**: Kim H. et al.
**摘要**: 该研究利用ALDH1B1抗体进行免疫荧光染色，发现ALDH1B1在人类胰腺导管细胞和胰岛祖细胞中特异性表达，提示其可作为胰腺干细胞标记物，并参与糖尿病相关再生过程。
2. **文献名称**: *ALDH1B1 regulates mitochondrial metabolism in colorectal cancer cells*
**作者**: Singh S. et al.
**摘要**: 通过Western blot和免疫组化技术(使用ALDH1B1抗体)，证实ALDH1B1在结直肠癌中高表达，其通过调控线粒体氧化代谢促进肿瘤细胞增殖，为潜在治疗靶点。
3. **文献名称**: *Aldehyde dehydrogenase 1B1 predicts chemotherapy resistance in gastric cancer*
**作者**: Chen Y. et al.
**摘要**: 研究采用ALDH1B1抗体检测胃癌组织，发现ALDH1B1高表达与5-FU化疗耐药显著相关，机制涉及ROS清除和DNA修复通路激活，可作为预后生物标志物。
注：以上为虚拟文献案例，实际研究中建议通过PubMed或Web of Science检索具体文献(关键词：ALDH1B1 antibody + cancer/stem cells/metabolism)。
ALDH1B1 (Aldehyde Dehydrogenase 1 Family Member B1) is a mitochondrial enzyme belonging to the aldehyde dehydrogenase superfamily, which plays a critical role in detoxifying endogenous and exogenous aldehydes by oxidizing them into carboxylic acids. It is primarily expressed in metabolic tissues, including the liver, pancreas, and intestinal mucosa, and is implicated in cellular processes such as oxidative stress response, retinoic acid synthesis, and stem cell maintenance. ALDH1B1 has gained attention for its potential involvement in cancer progression, particularly in tumors with stem-like properties, where it may regulate metabolic reprogramming and chemoresistance.
Antibodies targeting ALDH1B1 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Given the high sequence homology among ALDH family members, specificity validation is crucial to avoid cross-reactivity. Researchers often verify ALDH1B1 antibodies using knockout cell lines or tissues to confirm target selectivity. ALDH1B1-specific antibodies have facilitated discoveries linking its overexpression to poor prognosis in cancers like colorectal carcinoma and hepatocellular carcinoma, as well as its role in mitochondrial dysfunction and metabolic disorders. Commercial antibodies are typically raised against unique peptide sequences within the ALDH1B1 protein, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) varying by supplier. Proper controls and validation remain critical for reliable experimental outcomes.
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