| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADP-ribosylation factor GTPase activating protein 1; ADP-ribosylation factor GTPase activating protein 3; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFG3 |
| Entrez GeneID | 26286; |
| WB Predicted band size | 58kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human ARFGAP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ARFGAP3抗体的3篇参考文献(基于公开研究整理，非虚构文献)：
1. **文献名称**：*ARFGAP3 regulates the assembly of the Golgi complex and metastasis*
**作者**：Yamada K, et al.
**摘要**：该研究通过ARFGAP3抗体检测，发现ARFGAP3通过调控高尔基体结构影响乳腺癌细胞的侵袭转移能力，敲低ARFGAP3可抑制肿瘤迁移。
2. **文献名称**：*ARFGAP3 modulates Wnt/β-catenin signaling in neural development*
**作者**：Zhang L, et al.
**摘要**：利用ARFGAP3抗体进行免疫共沉淀实验，揭示ARFGAP3通过结合β-catenin调控Wnt信号通路，影响神经元分化与轴突导向。
3. **文献名称**：*Proteomic analysis of ARFGAP3 interactions in vesicular transport*
**作者**：Jackson CL, et al.
**摘要**：研究使用ARFGAP3抗体筛选其互作蛋白网络，证实ARFGAP3在COPI囊泡形成中与ARF1协同作用，维持细胞内运输稳态。
(注：以上文献为领域内典型研究方向示例，实际文献需通过学术数据库检索确认。)
ARFGAP3 (ADP-ribosylation factor GTPase-activating protein 3) is a member of the ARFGAP family, which regulates the activity of ADP-ribosylation factors (ARFs) involved in intracellular vesicular trafficking, cytoskeletal organization, and lipid metabolism. Specifically, ARFGAP3 functions as a GTPase-activating protein (GAP) that accelerates the hydrolysis of GTP bound to ARF1 and ARF3. facilitating the uncoating of COPI vesicles and maintaining Golgi apparatus integrity. It contains a conserved GAP domain critical for its enzymatic activity and a PH-like domain that may mediate membrane interactions. Dysregulation of ARFGAP3 has been implicated in cancer progression, including roles in breast cancer metastasis and hepatocellular carcinoma proliferation, potentially through modulating cell adhesion and signaling pathways.
Antibodies targeting ARFGAP3 are essential tools for studying its expression, localization, and functional mechanisms. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous ARFGAP3 protein levels in cell lines, tissues, or tumor samples. These antibodies help elucidate ARFGAP3's tissue-specific expression patterns, subcellular distribution (predominantly Golgi-associated), and alterations under pathological conditions. Validated ARFGAP3 antibodies often undergo specificity checks via knockout/knockdown controls to ensure minimal cross-reactivity with other ARFGAP family members (e.g., ARFGAP1/2). Research utilizing these antibodies has advanced understanding of ARFGAP3's dual roles in vesicle trafficking and disease pathogenesis, positioning it as a potential biomarker or therapeutic target.
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