| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | adenine nucleotide translocator 4; ADP; ATP carrier protein 4; ADP/ATP translocase 4; ANT 4 |
| Entrez GeneID | 83447; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human SLC25A31. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SLC25A31抗体的参考文献(示例基于文献内容模拟，非真实存在)：
1. **文献名称**: "SLC25A31 modulates mitochondrial apoptosis by regulating adenine nucleotide transport"
**作者**: Zhang L, et al.
**摘要**: 本研究利用SLC25A31特异性抗体，通过Western blot和免疫荧光证实其在HeLa细胞线粒体中的定位，并发现其表达下调会抑制ATP/ADP交换，进而激活caspase依赖性凋亡通路。
2. **文献名称**: "Commercial SLC25A31 antibody validation for immunohistochemical applications in breast cancer tissues"
**作者**: Thompson R, et al.
**摘要**: 文章系统评估了多种商业来源的SLC25A31抗体(如Abcam #xxxx和Santa Cruz #yyy)在乳腺癌组织中的特异性，发现部分抗体存在交叉反应性，推荐使用抗原肽预吸附法提高检测准确性。
3. **文献名称**: "SLC25A31 as a metabolic biomarker in non-alcoholic fatty liver disease: Insights from murine models"
**作者**: Gupta S, et al.
**摘要**: 通过SLC25A31抗体检测小鼠肝脏中线粒体腺苷酸转运蛋白水平，发现其表达与脂肪堆积程度呈负相关，提示其在脂质代谢中的潜在调控作用。
4. **文献名称**: "CRISPR/Cas9-mediated SLC25A31 knockout alters oxidative phosphorylation in cardiomyocytes"
**作者**: Chen H, et al.
**摘要**: 使用SLC25A31抗体验证基因敲除效率，并发现缺失该蛋白会导致心肌细胞线粒体膜电位下降及ROS累积，表明其对心脏能量稳态的关键性。
(注：以上为模拟文献，实际引用需查询PubMed、Google Scholar等平台。)
The SLC25A31 antibody targets the solute carrier family 25 member 31 (SLC25A31), a mitochondrial carrier protein primarily involved in adenosine nucleotide transport across the mitochondrial inner membrane. Also known as adenine nucleotide translocase 4 (ANT4), SLC25A31 facilitates the exchange of ATP and ADP, critical for maintaining cellular energy homeostasis. It is encoded by the SLC25A31 gene and is predominantly expressed in germ cells, particularly during spermatogenesis, highlighting its role in male fertility. Dysregulation of SLC25A31 has been implicated in mitochondrial disorders, metabolic syndromes, and certain cancers, including leukemia and hepatocellular carcinoma.
Antibodies against SLC25A31 are essential tools for studying its expression, localization, and function in cellular models and tissues. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate mitochondrial dynamics, energy metabolism, and disease mechanisms. Commercially available antibodies are typically validated for specificity using knockout controls or siRNA-mediated silencing. Research applications span reproductive biology, cancer metabolism, and neurodegenerative diseases linked to mitochondrial dysfunction. The protein’s structure—approximately 300 amino acids with three transmembrane domains—is often analyzed using epitope-specific antibodies to explore conformational changes or post-translational modifications.
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