| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | acyl-CoA thioesterase 4; acyl-coenzyme A thioesterase 4; EC 3.1.2.2; peroxisomal acyl coenzyme A thioester hydrolase Ib; peroxisomal long-chain acyl-CoA thioesterase Ib |
| Entrez GeneID | 122970; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ACOT4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACOT4抗体的3篇参考文献示例(注：以下内容为模拟生成，非真实文献)：
1. **文献名称**：*Characterization of ACOT4 Enzyme Activity and Antibody Specificity in Human Liver Tissue*
**作者**：Smith JL, et al.
**摘要**：本研究通过制备多克隆ACOT4抗体，验证了其在人肝组织中的特异性表达，并发现ACOT4在脂肪酸氧化途径中调控中链酰基辅酶A的代谢活性。
2. **文献名称**：*ACOT4 Expression in Pancreatic β-Cells: Role in Lipotoxicity and Diabetes*
**作者**：Chen R, et al.
**摘要**：利用ACOT4特异性抗体进行免疫荧光染色，揭示了ACOT4在胰岛β细胞中的定位，并证明其表达水平升高与脂毒性诱导的胰岛素分泌功能障碍相关。
3. **文献名称**：*Development of a Monoclonal Antibody for ACOT4 with Applications in Cancer Metabolism Studies*
**作者**：Wang H, et al.
**摘要**：报道了一种新型单克隆ACOT4抗体的开发，通过Western blot和免疫组化验证其在肿瘤组织中的反应性，并发现ACOT4在结直肠癌中高表达且与不良预后相关。
(如需真实文献，建议通过PubMed或Google Scholar以“ACOT4 antibody”为关键词检索近期研究。)
The ACOT4 (Acyl-CoA Thioesterase 4) antibody is a tool used to study the function and expression of the ACOT4 enzyme, a member of the acyl-CoA thioesterase family. ACOT4 catalyzes the hydrolysis of acyl-CoA thioesters into free fatty acids and coenzyme A (CoASH), regulating lipid metabolism and cellular signaling. This enzyme is thought to localize to peroxisomes or mitochondria, where it may influence β-oxidation pathways by modulating acyl-CoA availability. ACOT4 shows substrate specificity for medium- to long-chain acyl-CoAs, distinguishing it from other ACOT isoforms. Its expression is prominent in metabolic tissues, including the liver, kidney, and adipose tissue, suggesting roles in energy homeostasis and lipid processing.
Research on ACOT4 has linked it to metabolic disorders, cancer, and neurodegenerative diseases, as dysregulation of lipid metabolism is a hallmark of these conditions. The ACOT4 antibody enables detection and quantification of ACOT4 protein levels in experimental models, aiding studies on its enzymatic activity, tissue distribution, and interaction partners. Validated applications include Western blotting, immunohistochemistry, and immunofluorescence. Specificity is critical, as cross-reactivity with other ACOT family members (e.g., ACOT1. ACOT2) can occur; thus, rigorous validation using knockout controls or siRNA knockdown is recommended. This antibody serves as a key reagent in exploring ACOT4's physiological and pathological roles, particularly in lipid-associated disease mechanisms.
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