| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Acyl-coenzyme A thioesterase 12; EC 3.1.2.1; Acyl-CoA thioesterase 12; Acyl-CoA thioester hydrolase 12; Cytoplasmic acetyl-CoA hydrolase 1 |
| Entrez GeneID | 134526; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ACOT12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACOT12抗体的3篇文献示例(内容基于假设性研究，实际文献需通过数据库验证)：
1. **文献名称**: "ACOT12 regulates lipid metabolism and promotes hepatocellular carcinoma progression"
**作者**: Li X, et al.
**摘要**: 该研究利用ACOT12特异性抗体，发现ACOT12在肝癌细胞中高表达，并通过调控脂肪酸氧化促进肿瘤生长。实验通过Western blot和免疫组化验证了ACOT12的蛋白表达水平与患者预后相关。
2. **文献名称**: "Characterization of a novel polyclonal antibody against human ACOT12 and its application in metabolic studies"
**作者**: Smith J, et al.
**摘要**: 研究团队开发了一种针对人源ACOT12的多克隆抗体，验证了其特异性及在细胞和组织中的定位。通过免疫沉淀和质谱分析，揭示了ACOT12与线粒体脂肪酸代谢途径的相互作用。
3. **文献名称**: "ACOT12 as a potential biomarker for obesity-related metabolic dysfunction"
**作者**: Wang Y, et al.
**摘要**: 该文献利用ACOT12抗体检测脂肪组织中ACOT12的表达水平，发现其与肥胖患者胰岛素抵抗显著相关，提示ACOT12可能成为代谢综合征的治疗靶点。
**备注**：以上文献信息为示例性质，实际引用需通过PubMed、Google Scholar等平台检索真实文献(关键词：ACOT12 antibody, ACOT12 function)。
The ACOT12 (Acyl-CoA Thioesterase 12) antibody is a tool used to study the function and expression of the ACOT12 enzyme, which belongs to the acyl-CoA thioesterase family. ACOT12 catalyzes the hydrolysis of acyl-CoA molecules into free fatty acids and coenzyme A, playing a critical role in regulating intracellular lipid metabolism, energy balance, and cellular signaling. It is particularly involved in mitochondrial fatty acid metabolism, influencing processes such as β-oxidation and lipid biosynthesis.
Research has linked ACOT12 dysregulation to metabolic disorders and cancers. For example, its overexpression has been observed in hepatocellular carcinoma (HCC), where it may promote tumor growth by altering lipid metabolism and supporting cancer cell proliferation. ACOT12 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression levels in tissues or cell lines. These studies help elucidate ACOT12's role in disease mechanisms, potential as a diagnostic biomarker, or therapeutic target.
Antibodies targeting ACOT12 are typically raised in hosts like rabbits or mice, with validation for specificity and sensitivity across experimental models. Ongoing research continues to explore its interaction networks and therapeutic implications in metabolic syndromes and oncology.
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