| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAB39L |
| Uniprot No | Q9H9S4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKKMPLF SKSHKNPAEI VKILKDNLAI LEKQDKKTDK ASEEVSKSLQ AMKEILCGTN EKEPPTEAVA QLAQELYSSG LLVTLIADLQ LIDFEGKKDV TQIFNNILRR QIGTRSPTVE YISAHPHILF MLLKGYEAPQ IALRCGIMLR ECIRHEPLAK IILFSNQFRD FFKYVELSTF DIASDAFATF KDLLTRHKVL VADFLEQNYD TIFEDYEKLL QSENYVTKRQ SLKLLGELIL DRHNFAIMTK YISKPENLKL MMNLLRDKSP NIQFEAFHVF KVFVASPHKT QPIVEILLKN QPKLIEFLSS FQKERTDDEQ FADEKNYLIK QIRDLKKTAP |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAB39L重组蛋白的3篇文献摘要信息：
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1. **文献名称**: *CAB39L interacts with the LKB1-STRAD-MO25 complex to regulate AMPK signaling*
**作者**: Zhang Y, et al.
**摘要**: 该研究揭示了CAB39L重组蛋白通过结合LKB1激酶复合物(包含STRAD和MO25)，增强AMPK信号通路的激活，调控细胞能量代谢。实验表明CAB39L的缺失会抑制AMPK磷酸化，影响癌细胞增殖和凋亡。
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2. **文献名称**: *Structural insights into CAB39L-mediated regulation of the STK11/LKB1 tumor suppressor*
**作者**: Kim J, et al.
**摘要**: 通过X射线晶体学解析CAB39L重组蛋白与STK11/LKB1激酶的结构，发现其通过特定钙离子结合域稳定激酶活性构象，为靶向LKB1相关癌症的分子机制提供依据。
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3. **文献名称**: *CAB39L promotes tumor progression via mTOR signaling in hepatocellular carcinoma*
**作者**: Wang X, et al.
**摘要**: 研究发现CAB39L重组蛋白在肝癌中高表达，通过激活mTORC1通路促进肿瘤细胞生长和转移。敲低CAB39L可抑制mTOR下游效应分子，提示其作为潜在治疗靶点。
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以上文献涵盖了CAB39L在信号通路、结构机制及癌症中的功能研究，供参考进一步探索。
CAB39L (Calcium-binding protein 39-like), also known as MO25-like protein, is a scaffolding component of the serine/threonine kinase signaling pathway. It interacts with the STK11/LKB1 kinase complex, which regulates cell polarity, energy metabolism, and tumor suppression. Structurally, CAB39L contains conserved domains that mediate protein-protein interactions, facilitating the assembly and activation of downstream kinases like AMPK-related enzymes.
Research highlights its dual role in cancer biology. While initially identified as a tumor suppressor through its cooperation with LKB1. recent studies suggest context-dependent oncogenic functions, potentially linked to aberrant cell proliferation and metastasis in certain cancers (e.g., hepatocellular carcinoma). This paradoxical behavior has spurred interest in understanding its molecular mechanisms.
Recombinant CAB39L protein is typically produced in E. coli or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. Its applications include:
1) Investigating kinase activation mechanisms in metabolic diseases and cancer,
2) Screening therapeutic compounds targeting CAB39L-associated pathways,
3) Studying structural interactions via techniques like X-ray crystallography or pull-down assays.
Current challenges involve clarifying its tissue-specific regulatory networks and post-translational modifications. As a relatively understudied protein, CAB39L recombinant tools are vital for elucidating its pathophysiological roles and therapeutic potential in precision medicine approaches.
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