| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABD12; ABHD12; abhydrolase domain containing 12; |
| Entrez GeneID | 26090; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human ABHD12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ABHD12抗体的3篇参考文献，包含文献名称、作者及摘要概括：
1. **文献名称**：*Mutations in ABHD12 cause the neurodegenerative disease PHARC*
**作者**：Fiskerstrand T, et al.
**摘要**：本研究首次将ABHD12基因突变与PHARC综合征(多发性神经病变、听力损失、共济失调等)相关联。研究利用特异性抗体检测患者细胞中ABHD12蛋白的表达缺失，证实突变导致功能丧失，为疾病机制提供了分子证据。
2. **文献名称**：*ABHD12 regulates brain lysophosphatidylserine pathways in a mouse model of neurodegenerative disease*
**作者**：Blankman JL, et al.
**摘要**：通过构建ABHD12基因敲除小鼠模型，研究揭示了ABHD12在降解脂质信号分子lysophosphatidylserine中的关键作用。研究中采用ABHD12抗体进行Western blot和免疫组化分析，证实蛋白在中枢神经系统的广泛表达，并探讨其缺失引发的神经炎症表型。
3. **文献名称**：*Structural and functional characterization of human ABHD12 as a lysophosphatidylserine lipase*
**作者**：Tian Y, et al.
**摘要**：该研究解析了ABHD12的酶活性底物特异性，证实其作为lysophosphatidylserine水解酶的功能。通过开发高特异性抗体，研究团队验证了ABHD12在细胞膜定位及其在脂质代谢通路中的调控作用，为靶向治疗提供了理论基础。
4. **文献名称**：*ABHD12 deficiency causes microglial activation via dysregulation of lysosomal phosphatidylserine metabolism*
**作者**：Yamasaki T, et al.
**摘要**：本研究利用ABHD12抗体进行免疫荧光和流式细胞分析，发现ABHD12缺失导致小胶质细胞异常激活及溶酶体脂质代谢紊乱。结果提示ABHD12通过维持脂质稳态抑制神经炎症，其抗体在病理模型中的应用为疾病机制研究提供了工具支持。
以上文献涵盖了ABHD12抗体的开发、验证及其在疾病机制与功能研究中的应用，涉及基因突变分析、蛋白表达检测及代谢调控机制探索。
ABHD12 (α/β-hydrolase domain-containing protein 12) is a serine hydrolase enzyme encoded by the *ABHD12* gene, primarily expressed in the brain, immune cells, and other tissues. It plays a critical role in lipid metabolism, particularly in hydrolyzing bioactive lipids such as lysophosphatidylserine (lyso-PS) and 2-arachidonoylglycerol (2-AG), which are involved in inflammatory signaling and endocannabinoid system regulation. Mutations in *ABHD12* are linked to the neurodegenerative disorder PHARC (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataracts), highlighting its importance in neurological function.
ABHD12 antibodies are immunological tools designed to detect and quantify the ABHD12 protein in research and diagnostic contexts. These antibodies are essential for studying ABHD12's expression patterns, subcellular localization, and interaction partners. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to investigate its role in lipid homeostasis, neuroinflammation, and neurodegenerative diseases. Additionally, ABHD12 antibodies aid in exploring potential therapeutic targets, as dysregulation of ABHD12 activity is implicated in conditions like multiple sclerosis, Alzheimer’s disease, and metabolic disorders. Validation of these antibodies is crucial to ensure specificity, given the structural similarities among serine hydrolases. Overall, ABHD12 antibodies serve as vital reagents for advancing our understanding of ABHD12’s biological functions and its association with human diseases.
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