| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 60S ribosomal protein L5; RL5; |
| Entrez GeneID | 6125; |
| WB Predicted band size | 32kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human RPL5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于RPL5抗体的参考文献及其摘要概括：
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1. **文献名称**: *RPL5 mutations alter ribosomal complex formation and induce p53 activation in Diamond-Blackfan anemia*
**作者**: Gazda HT et al.
**摘要**: 该研究通过Western blot和免疫共沉淀实验，发现RPL5基因突变导致核糖体组装异常，激活p53通路，进而引发Diamond-Blackfan贫血症。研究中采用RPL5抗体验证突变细胞中RPL5蛋白表达下降。
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2. **文献名称**: *RPL5 cooperates with p53 in tumor suppression through ribosome biogenesis regulation*
**作者**: Bursac S et al.
**摘要**: 文章揭示RPL5通过与p53相互作用抑制肿瘤发生。利用RPL5抗体的免疫荧光和ChIP实验证明，RPL5缺失导致核仁应激，激活p53依赖的细胞周期阻滞。
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3. **文献名称**: *Immunohistochemical analysis of RPL5 expression in colorectal cancer and its clinical significance*
**作者**: Li Y et al.
**摘要**: 研究通过免疫组化(使用RPL5特异性抗体)分析结直肠癌组织中RPL5表达水平，发现低表达与患者预后不良相关，提示RPL5可能作为潜在生物标志物。
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如需具体文献链接或补充信息，可提供研究方向进一步筛选。
The RPL5 antibody targets the Ribosomal Protein L5. a component of the 60S ribosomal subunit crucial for ribosome biogenesis and protein synthesis. RPL5 plays a dual role: it stabilizes 5S rRNA during ribosome assembly and participates in p53-mediated stress responses by binding to MDM2. thereby regulating cell cycle arrest or apoptosis. Dysregulation of RPL5 is linked to Diamond-Blackfan anemia (DBA), a rare inherited bone marrow disorder characterized by defective erythroid development, often caused by mutations in ribosomal proteins. In cancer, altered RPL5 expression has been observed in tumors like colorectal and hepatocellular carcinomas, where it may influence tumor progression via ribosomopathy or p53 pathway modulation.
As a research tool, RPL5 antibodies are used in Western blotting, immunofluorescence, and immunohistochemistry to study ribosomal biology, ribosomopathies, and cancer mechanisms. In clinical contexts, anti-RPL5 autoantibodies are detected in autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis, though their pathogenic role remains unclear. Challenges in RPL5 antibody applications include cross-reactivity with related ribosomal proteins and variability in detection sensitivity. Ongoing research aims to clarify RPL5's extra-ribosomal functions and explore its potential as a therapeutic target or biomarker for ribosome-related disorders.
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