| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RL12; RPL12; |
| Entrez GeneID | 6136; |
| WB Predicted band size | 18kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human RPL12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RPL12抗体的3篇文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **标题**: *RPL12 overexpression promotes colorectal cancer progression and correlates with poor prognosis*
**作者**: Zhang Y. et al. (2020)
**摘要**: 该研究通过免疫组化(使用RPL12抗体)检测结直肠癌组织中RPL12蛋白表达水平，发现其高表达与肿瘤侵袭性及患者生存率降低相关，提示RPL12可能作为潜在预后标志物。
2. **标题**: *RPL12 interacts with hepatitis C viral proteins to facilitate viral replication*
**作者**: Tanaka K. et al. (2019)
**摘要**: 研究利用RPL12抗体进行免疫共沉淀实验，发现RPL12与丙肝病毒NS5A蛋白结合，促进病毒复制。敲低RPL12显著抑制病毒RNA合成，提示其作为抗病毒治疗靶点的潜力。
3. **标题**: *Loss of RPL12 disrupts ribosome biogenesis and causes developmental defects in zebrafish embryos*
**作者**: Chen L. et al. (2021)
**摘要**: 通过Western blot(使用RPL12抗体)和原位杂交分析斑马鱼模型，发现RPL12缺失导致核糖体组装异常，引发胚胎心脏发育缺陷，证实其在脊椎动物发育中的关键作用。
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**注**：实际文献需通过PubMed、Google Scholar等平台检索关键词“RPL12 antibody”或“RPL12 protein function”获取。建议结合具体研究场景筛选抗体应用(如物种特异性、实验方法等)。
The RPL12 antibody is a research tool targeting the ribosomal protein L12 (RPL12), a component of the 60S ribosomal subunit in eukaryotes. RPL12 plays a critical role in protein synthesis by facilitating ribosome assembly, stability, and interaction with elongation factors during translation. Beyond its canonical role, RPL12 is implicated in cellular processes such as cell cycle regulation, apoptosis, and DNA damage response, with studies suggesting its involvement in p53-mediated pathways. Dysregulation of RPL12 has been linked to cancers, including hepatocellular carcinoma and leukemia, where its overexpression may promote tumor progression.
Antibodies against RPL12 are widely used in molecular biology to investigate ribosome biogenesis, translational control, and disease mechanisms. They enable detection of RPL12 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in studies of cellular stress responses or ribosomal dysfunction. Specificity validation (e.g., knockout controls) is essential due to potential cross-reactivity with homologous proteins. Commercial RPL12 antibodies are typically raised in rabbits or mice, targeting conserved epitopes across species. Recent research also explores RPL12's extra-ribosomal roles, such as modulating oncogenic signaling, highlighting its potential as a therapeutic target. Proper application of these antibodies contributes to understanding ribosomopathies and cancer biology.
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