| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MGF; STAT5 |
| Entrez GeneID | 20850 |
| WB Predicted band size | Calculated MW: 91 kDa; Observed MW: 91 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of mouse STAT5A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MRPL44抗体的参考文献示例(注：以下内容为虚构示例，建议通过学术数据库查询真实文献)：
1. **文献名称**: "MRPL44 regulates mitochondrial translation and apoptosis in mammalian cells"
**作者**: Smith A, et al.
**摘要**: 本研究通过敲低和过表达MRPL44.发现其在维持线粒体核糖体功能及调控细胞凋亡中的关键作用。使用特异性MRPL44抗体进行Western blot和免疫荧光，证实其在线粒体内的定位及与凋亡相关蛋白的相互作用。
2. **文献名称**: "A novel mutation in MRPL44 causes mitochondrial dysfunction in patient-derived fibroblasts"
**作者**: Chen L, et al.
**摘要**: 报道一例由MRPL44基因突变引起的线粒体疾病病例。通过免疫组化(使用MRPL44抗体)和呼吸链活性分析，揭示突变导致线粒体蛋白合成缺陷及ATP生成减少。
3. **文献名称**: "Proteomic profiling of mitochondrial ribosomes identifies MRPL44 as a biomarker for cancer progression"
**作者**: Wang Y, et al.
**摘要**: 利用MRPL44抗体进行蛋白质组学分析，发现其在多种癌症组织中高表达，并与肿瘤代谢重编程相关。研究提示MRPL44可能作为癌症诊断的潜在标志物。
4. **文献名称**: "Functional characterization of MRPL44 in Drosophila models of neurodegenerative disease"
**作者**: Gonzalez R, et al.
**摘要**: 在果蝇模型中探究MRPL44的神经保护功能，通过抗体染色显示其表达异常与神经退行性病变中线粒体形态缺陷的相关性。
建议通过 **PubMed** 或 **Google Scholar** 搜索关键词 "MRPL44 antibody" 或 "MRPL44 function" 获取真实文献。
The MRPL44 antibody is a crucial tool for studying the mitochondrial ribosomal protein L44 (MRPL44), a component of the 39S large subunit of the mitochondrial ribosome. Mitochondrial ribosomes are responsible for translating 13 essential subunits of the oxidative phosphorylation (OXPHOS) complexes encoded by mitochondrial DNA, which are critical for cellular energy production. MRPL44 plays a structural or functional role in ribosome assembly, stability, or translation efficiency, though its precise mechanisms remain under investigation. Dysregulation of mitochondrial ribosome proteins, including MRPL44. has been linked to mitochondrial disorders, metabolic diseases, and cancer, as impaired OXPHOS can drive metabolic reprogramming and disease progression.
The MRPL44 antibody is typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant proteins. It enables detection and quantification of MRPL44 expression via techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Researchers use this antibody to explore MRPL44's tissue-specific expression, subcellular localization (mitochondrial matrix), and alterations under pathological conditions. Validated applications include studying mitochondrial translation defects, metabolic adaptations in cancer, and molecular mechanisms of mitochondrial dysfunction. Specificity is confirmed through knockdown/knout controls or mass spectrometry. Its utility spans basic mitochondrial biology, disease biomarker discovery, and therapeutic target validation.
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