| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 39S ribosomal protein L15; mitochondrial [Precursor]; L15mt; MRP-L15; HSPC145 |
| Entrez GeneID | 29088; |
| WB Predicted band size | 33kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human MRPL15. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Mitochondrial ribosomal protein L15 regulates cell cycle progression in colorectal cancer"**
- Author: Zhang et al. (2020)
- 摘要：研究通过Western blot和免疫组化分析MRPL15抗体在结直肠癌组织中的表达，发现其高表达与细胞周期调控异常及患者预后不良相关。
2. **"MRPL15 modulates mitochondrial translation and oxidative phosphorylation in mammalian cells"**
- Author: Lee et al. (2018)
- 摘要：利用MRPL15抗体进行免疫荧光和蛋白质组学分析，揭示MRPL15在线粒体核糖体组装和能量代谢中的关键作用，敲低后导致ATP合成受损。
3. **"A role for MRPL15 in hematopoietic stem cell differentiation via ribosome biogenesis"**
- Author: Smith et al. (2019)
- 摘要：通过流式细胞术和抗体染色证实MRPL15在造血干细胞中线粒体核糖体生物合成的调控功能，影响分化进程和细胞存活。
4. **"Proteomic identification of MRPL15 as a biomarker for hepatocellular carcinoma"**
- Author: Chen et al. (2021)
- 摘要：基于MRPL15抗体的组织芯片分析，发现其表达水平与肝癌进展相关，可能作为潜在诊断标志物和治疗靶点。
注：以上文献信息为模拟示例，实际引用时需核实原文。
The MRPL15 antibody is a research tool designed to detect and study the Mitochondrial Ribosomal Protein L15 (MRPL15), a key component of the mitochondrial ribosome's large (39S) subunit. Encoded by the nuclear genome and imported into mitochondria, MRPL15 plays a critical role in mitochondrial translation, which is essential for synthesizing subunits of the oxidative phosphorylation (OXPHOS) machinery. Dysregulation of mitochondrial ribosomes has been linked to metabolic disorders, neurodegenerative diseases, and cancer, making MRPL15 a target for investigating mitochondrial dysfunction.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to analyze MRPL15 expression, localization, and abundance across tissues or experimental conditions. Researchers employ it to explore mitochondrial protein synthesis efficiency, cellular stress responses, and pathologies associated with mitochondrial gene mutations. Validation typically includes testing in MRPL15-knockdown models or mitochondrial-enriched fractions to confirm specificity.
Commercial MRPL15 antibodies are often raised in rabbits or mice, with formats including monoclonal or polyclonal versions. Proper controls are crucial due to potential cross-reactivity with cytoplasmic ribosomal proteins. Its applications span basic mitochondrial biology, disease mechanism studies, and drug development targeting energy metabolism pathways.
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