| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 39S ribosomal protein 54; mitochondrial; L54mt; mitochondrial ribosomal protein L54; MRP-L54 |
| Entrez GeneID | 116541; |
| WB Predicted band size | 20kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human MRPL54. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPL54抗体的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*MRPL54 is a mitochondrial ribosome assembly factor involved in energy metabolism disorders*
**作者**：Smith A, et al.
**摘要**：本研究通过CRISPR筛选发现MRPL54是线粒体核糖体大亚基组装的关键因子。利用特异性抗体进行免疫印迹和免疫荧光实验，证实其缺失导致线粒体翻译缺陷和ATP合成减少，提示其与代谢疾病相关。
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2. **文献名称**：*Characterization of MRPL54 antibody for detecting mitochondrial dysfunction in cancer cells*
**作者**：Chen L, et al.
**摘要**：文章验证了一种商业化MRPL54抗体的特异性，证明其适用于免疫组化和流式细胞术。研究发现某些癌细胞中MRPL54表达下调与线粒体呼吸链活性降低相关，可能成为癌症治疗的潜在靶点。
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3. **文献名称**：*The role of MRPL54 in neurodegenerative diseases: Insights from antibody-based proteomic analysis*
**作者**：Wang Y, et al.
**摘要**：通过抗体介导的蛋白质组学分析，发现阿尔茨海默病患者脑组织中MRPL54表达异常。该抗体被用于验证线粒体核糖体功能障碍与神经退行性变之间的关联。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar以关键词“MRPL54 antibody”或“MRPL54 mitochondrial ribosome”检索最新文献，并优先选择高影响力期刊(如*Cell Metabolism*、*Nature Cell Biology*)的研究。部分研究可能未直接提及抗体开发，但会在方法学中描述其应用。
The MRPL54 antibody is a research tool designed to detect mitochondrial ribosomal protein L54 (MRPL54), a component of the mitochondrial large ribosomal subunit. Mitochondrial ribosomes are responsible for translating mitochondrial DNA-encoded mRNAs into proteins essential for oxidative phosphorylation (OXPHOS), the primary energy-producing pathway in eukaryotic cells. MRPL54. encoded by the nuclear gene MRPL54. contributes to ribosome assembly and stability, ensuring proper synthesis of mitochondrial-encoded subunits of the electron transport chain complexes.
Dysregulation of mitochondrial ribosome proteins, including MRPL54. has been linked to mitochondrial dysfunction, which is associated with metabolic disorders, neurodegenerative diseases, and cancer. MRPL54 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate MRPL54 expression levels, subcellular localization, and interactions in disease models or under stress conditions. These studies aim to elucidate the role of mitochondrial translation in cellular energy homeostasis, apoptosis, and disease pathogenesis.
Commercially available MRPL54 antibodies are typically validated for specificity in human, mouse, or rat samples. Researchers utilize them to explore mitochondrial protein synthesis defects, screen for biomarkers in mitochondrial diseases, or assess therapeutic responses targeting mitochondrial metabolism. Proper controls, such as gene knockdown or knockout samples, are recommended to confirm antibody specificity.
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