| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 28S ribosomal protein S12 mitochondrial; S12mt; MRP-S12; MT-RPS12; |
| Entrez GeneID | 6183; |
| WB Predicted band size | 20kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human MRPS12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPS12抗体的参考文献示例(注：以下文献为示例性内容，实际引用时需核实真实来源)：
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1. **"Development of a Polyclonal Antibody Against Human MRPS12 for Mitochondrial Proteomic Studies"**
*作者：Li X, et al.*
**摘要**：本研究报道了一种针对人源MRPS12蛋白的多克隆抗体的制备与验证。通过免疫印迹和免疫荧光实验，证实该抗体特异性识别线粒体中的MRPS12.并成功应用于线粒体提取物的蛋白质组学分析，揭示了MRPS12与其他核糖体蛋白的相互作用。
2. **"MRPS12 Overexpression in Breast Cancer Correlates with Poor Prognosis: An Immunohistochemical Analysis"**
*作者：Wang Y, et al.*
**摘要**：利用商业化抗MRPS12抗体对乳腺癌组织样本进行免疫组化检测，发现MRPS12在恶性肿瘤中显著高表达，且与患者生存率降低相关。研究提示MRPS12可能作为乳腺癌潜在的预后标志物。
3. **"Mitochondrial Dysfunction in Neurodegenerative Diseases: Role of MRPS12 and Antibody-Based Detection"**
*作者：Smith J, et al.*
**摘要**：通过抗MRPS12抗体分析阿尔茨海默病模型小鼠的脑组织，发现MRPS12表达水平降低与线粒体功能异常和神经元凋亡相关，为神经退行性疾病的机制研究提供了新视角。
4. **"Characterization of a Novel Monoclonal Antibody for MRPS12 in Zebrafish Developmental Studies"**
*作者：Garcia R, et al.*
**摘要**：开发了一种特异性识别斑马鱼MRPS12的单克隆抗体，并验证其在胚胎发育阶段的表达模式，证明MRPS12在线粒体生物发生中的关键作用。
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**注**：实际文献可能涉及更具体的应用场景(如疾病机制、诊断工具开发等)，建议通过学术数据库(如PubMed、Web of Science)以“MRPS12 antibody”为关键词检索最新研究。
The mitochondrial ribosomal protein S12 (MRPS12) is a component of the small subunit (28S) of the mitochondrial ribosome, which is essential for synthesizing proteins encoded by mitochondrial DNA (mtDNA). As part of the MRP family, MRPS12 plays a critical role in maintaining mitochondrial ribosome stability and facilitating translation of mtDNA-encoded subunits of oxidative phosphorylation (OXPHOS) complexes, which are vital for cellular energy production. Dysregulation of MRPS12 has been linked to mitochondrial dysfunction, contributing to diseases such as encephalopathy, cardiomyopathy, and metabolic disorders.
Antibodies targeting MRPS12 are widely used in research to study mitochondrial protein synthesis, ribosome assembly, and mitochondrial diseases. These antibodies enable detection of MRPS12 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry, helping to elucidate its tissue-specific distribution and subcellular localization within mitochondria. They are also employed in disease models to investigate correlations between MRPS12 mutations or altered expression and pathological phenotypes, such as impaired OXPHOS activity or abnormal mitochondrial morphology. Additionally, MRPS12 antibodies serve as tools for validating CRISPR/Cas9 knockouts or siRNA-mediated gene silencing in functional studies. Commercial MRPS12 antibodies are typically raised in hosts like rabbits or mice using synthetic peptides or recombinant proteins, with validation in specificity assays critical to ensure reliability. Their application advances understanding of mitochondrial biology and potential therapeutic targets for mitochondrial disorders.
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