| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | POU domain class 4 transcription factor 3; Brain-specific homeobox/POU domain protein 3C; Brn-3C; POU4F3; BRN3C |
| Entrez GeneID | 5459; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human POU4F3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POU4F3抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*POU4F3 mutation screening in Japanese hearing loss patients*
**作者**：Miyagawa M, Nishio SY, Usami SI
**摘要**：本研究通过基因测序分析日本耳聋患者的POU4F3突变情况，并利用POU4F3抗体进行Western blot验证突变蛋白的表达缺失，揭示了该基因突变与常染色体显性非综合征性耳聋的关联。
2. **文献名称**：*The role of POU4F3 in the maturation and survival of cochlear hair cells*
**作者**：Xiang M, Gan L, Li H
**摘要**：通过在小鼠模型中结合POU4F3抗体的免疫荧光染色，研究发现POU4F3对耳蜗毛细胞的发育和存活至关重要，其缺失导致进行性听力损失，提示其在毛细胞分化中的转录调控作用。
3. **文献名称**：*Immunohistochemical localization of POU4F3 in human inner ear tissues*
**作者**：Chen Y, Li R, Liu H
**摘要**：利用人内耳组织切片进行POU4F3抗体的免疫组化分析，首次证实POU4F3蛋白在成人耳蜗螺旋神经节神经元中的特异性表达，支持其在听觉信号传导中的潜在功能。
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**备注**：上述文献为示例性内容，实际引用时需核实具体文献来源及准确性。如需真实文献，建议通过PubMed或Google Scholar以关键词“POU4F3 antibody”或“POU4F3 hearing loss”检索近期研究。
The POU4F3 antibody targets the POU4F3 protein, a critical transcription factor encoded by the *POU4F3* gene (also known as *BRN3C*). This protein belongs to the POU-domain family, characterized by a conserved DNA-binding domain that regulates gene expression by binding to specific promoter regions. POU4F3 plays a vital role in the development and survival of sensory hair cells in the inner ear, which are essential for auditory function. Mutations in *POU4F3* are linked to autosomal dominant nonsyndromic hearing loss (DFNA15), highlighting its importance in maintaining hearing integrity.
Antibodies against POU4F3 are widely used in research to study its expression, localization, and function in cellular and tissue contexts. They enable detection via techniques like immunohistochemistry, Western blotting, and immunofluorescence, aiding in investigations of inner ear biology, neurodegenerative pathways, and genetic hearing disorders. Additionally, these antibodies help explore POU4F3's potential role in other systems, such as retinal ganglion cell development and certain cancers, where its dysregulation has been observed. By elucidating POU4F3's mechanisms, researchers aim to advance therapeutic strategies for hearing loss and related conditions.
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