| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog |
| Entrez GeneID | 5885; |
| WB Predicted band size | 72kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human RAD21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAD21抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Cohesin subunit RAD21: From biology to disease*
**作者**：Peters JM, Tedeschi A, Schmitz J
**摘要**：该综述系统总结了RAD21作为黏连蛋白核心亚基的功能，包括其在姐妹染色单体结合、DNA损伤修复及基因转录调控中的作用，并讨论了使用特异性抗体在染色质免疫沉淀(ChIP)和免疫荧光中的实验应用。
2. **文献名称**：*RAD21 alterations in acute myeloid leukemia: Clinical implications and genomic instability*
**作者**：Thota S, Viny AD, Makishima H, et al.
**摘要**：研究通过Western blot和免疫组化发现，急性髓系白血病(AML)患者中RAD21蛋白表达异常升高，且与基因组不稳定性和不良预后相关，提示其作为潜在治疗靶点的价值。
3. **文献名称**：*RAD21 loss disrupts cohesin complex integrity and leads to cellular senescence in human fibroblasts*
**作者**：Remeseiro S, Cuadrado A, Carretero M, et al.
**摘要**：利用RAD21抗体敲低实验，发现RAD21缺失会导致黏连蛋白复合体解体，引发DNA损伤应答和细胞衰老，揭示了其在维持基因组稳定性中的关键作用。
(注：以上文献为示例，实际引用时请核实具体信息。)
The RAD21 antibody targets a key component of the cohesin complex, a multiprotein structure essential for sister chromatid cohesion, DNA repair, and transcriptional regulation. RAD21 (also known as SCC1) forms a tripartite ring with SMC1 and SMC3. mediating the physical tethering of sister chromatids during cell division to ensure accurate chromosome segregation. Beyond mitosis, RAD21 participates in DNA damage repair, chromatin organization, and gene expression modulation by facilitating loop formation between regulatory elements and promoters. Dysregulation of RAD21 is linked to genomic instability, cancer progression (e.g., breast and colorectal cancers), and developmental disorders like Cornelia de Lange syndrome.
RAD21 antibodies are widely used in research to study cohesin dynamics, chromatin architecture (via ChIP-seq), and cell cycle mechanisms. They are critical tools for detecting RAD21 expression levels in tissues or cultured cells through techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Commercially available antibodies are often validated for specificity using knockout cell lines or siRNA knockdown. Researchers should select clones (e.g., EPR4535. D6T9Q) based on the application and species reactivity (human, mouse, rat). Recent studies also explore RAD21's role in 3D genome organization and its potential as a therapeutic target in cohesin-mutant cancers. Proper controls, like isotype-matched IgG and verification of protein size (~120 kDa), are essential to ensure reliable results.
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