Rabbit Polyclonal MLH3 Antibody

The MLH3 antibody is a crucial tool in studying the MLH3 protein, a component of the DNA mismatch repair (MMR) system. MLH3. a member of the MutL homolog family, forms heterodimers with MLH1 to participate in correcting DNA replication errors, particularly during meiosis. It plays a role in maintaining genomic stability and is implicated in hereditary cancers, notably Lynch syndrome (hereditary non-polyposis colorectal cancer, HNPCC), where MLH3 mutations may contribute to microsatellite instability (MSI) and tumorigenesis. MLH3 antibodies are widely used in research to detect protein expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These applications help investigate MMR deficiency, assess cancer risk, and validate MLH3's interaction partners. Antibodies targeting specific domains (e.g., C-terminal) are often validated using knockout cell lines to ensure specificity. Beyond research, MLH3 antibodies hold diagnostic potential in profiling MMR status in tumors, aiding Lynch syndrome screening. However, MLH3's functional overlap with other MutL proteins (e.g., PMS2) requires careful interpretation of results. Ongoing studies explore its role beyond MMR, including apoptosis and immune response, highlighting its broader biomedical relevance.