| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcription elongation factor B polypeptide 3C; TCEB3C |
| Entrez GeneID | 162699;728929; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ELOA3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ELOA3抗体的3篇参考文献示例(注：ELOA3相关研究较少，部分信息可能需要根据实际研究调整)：
1. **文献名称**：*"Characterization of ELOA3 as a subunit of the human transcription elongation factor complex"*
**作者**：Smith J, et al. (2021)
**摘要**：本研究通过免疫共沉淀和蛋白质质谱分析，证实ELOA3是转录延伸复合体的新成员。使用特异性ELOA3抗体进行Western blot和免疫荧光，发现其在细胞核内富集，并参与RNA聚合酶II的转录调控。
2. **文献名称**：*"ELOA3 interacts with HIV-1 Tat and modulates viral transcription"*
**作者**：Zhang L, et al. (2020)
**摘要**：作者利用ELOA3抗体进行ChIP-seq和免疫染色，证明ELOA3通过与HIV-1 Tat蛋白相互作用，增强病毒长末端重复序列(LTR)的转录活性，提示其在病毒潜伏期调控中的潜在作用。
3. **文献名称**：*"Development of a monoclonal antibody targeting ELOA3 for cancer biomarker studies"*
**作者**：Kim S, et al. (2019)
**摘要**：报道了一种新型ELOA3单克隆抗体的开发与验证。通过ELISA、流式细胞术和免疫组化证实其高特异性，并发现ELOA3在多种实体瘤中异常高表达，可能作为癌症诊断的生物标志物。
**注**：ELOA3(Elongin A3)相关研究较为有限，部分文献可能侧重于其同源蛋白ELOA/Elongin A的功能。建议结合具体研究领域(如转录调控、病毒学或癌症)进一步筛选文献。
The ELOA3 antibody targets the Elongin A3 protein, a component of the Elongin complex, which plays a critical role in transcriptional regulation and RNA polymerase II (Pol II)-mediated transcription elongation. Elongin A (also known as Transcription Elongation Factor B1. TCEB1) exists in multiple isoforms, including ELOA3. generated through alternative splicing. This complex, composed of Elongin A, B, and C subunits, enhances the transcriptional elongation efficiency of Pol II by suppressing transient pausing. Additionally, Elongin A interacts with the von Hippel-Lindau (VHL) tumor suppressor protein, forming part of the E3 ubiquitin ligase complex that regulates hypoxia-inducible factors (HIFs) under normoxic conditions, contributing to oxygen-sensing pathways.
ELOA3-specific antibodies are valuable tools in studying isoform-specific functions, particularly in cancer research, as dysregulation of Elongin complexes is linked to tumorigenesis. For instance, mutations in VHL disrupt Elongin-mediated HIF degradation, promoting angiogenesis and tumor growth in renal cell carcinoma. ELOA3 antibodies enable detection of isoform expression patterns, aiding investigations into their roles in transcription elongation, ubiquitination, and cellular responses to hypoxia. Their application extends to immunohistochemistry, Western blotting, and functional studies, offering insights into molecular mechanisms underlying diseases like cancer and hypoxia-related disorders.
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