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Rabbit Polyclonal ELOA1 Antibody

  • 中文名: ELOA1抗体
  • 别    名: Transcription elongation factor B polypeptide 3; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Elongin-A; EloA
货号: IPDX42207
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTranscription elongation factor B polypeptide 3; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Elongin-A; EloA
Entrez GeneID6924;
WB Predicted band size87kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenSynthesized peptide derived from internal of human ELOA1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于ELOA1抗体的参考文献示例(注:部分文献信息为示例性概括,实际引用请核实原文):

1. **文献名称**:*ELOA1 modulates RNA polymerase II dynamics during transcriptional elongation*

**作者**:Smith J, et al.

**摘要**:研究利用ELOA1特异性抗体进行染色质免疫共沉淀(ChIP),揭示了ELOA1在RNA聚合酶II延伸过程中的调控作用及其与转录复合物的相互作用。

2. **文献名称**:*Dysregulation of Elongin A (ELOA1) in renal cell carcinoma and its clinical implications*

**作者**:Lee H, et al.

**摘要**:通过ELOA1抗体进行免疫组化分析,发现ELOA1在肾癌细胞中表达显著下调,提示其可能作为肿瘤抑制因子参与癌症进展。

3. **文献名称**:*Structural insights into the Elongin complex assembly mediated by ELOA1*

**作者**:Zhang R, et al.

**摘要**:使用ELOA1抗体进行免疫共沉淀和Western blot,解析了ELOA1与Elongin B/C复合物的结构,阐明了其在转录延伸中的分子机制。

4. **文献名称**:*ELOA1 antibody validation for flow cytometry applications*

**作者**:Garcia M, et al.

**摘要**:验证了ELOA1抗体在流式细胞术中的特异性,并应用于检测多种细胞系中ELOA1的蛋白表达水平及亚细胞定位。

(注:上述文献为假设性示例,实际研究中请通过PubMed、Web of Science等平台检索具体文献。)

背景信息

**Background of ELOA1 Antibody**

The ELOA1 (Elongin A) antibody targets the ELOA1 protein, a critical subunit of the Elongin complex, which plays a vital role in transcriptional regulation and RNA polymerase II-mediated elongation. ELOA1. also known as transcription elongation factor B subunit 1 (TCEB1), forms a heterotrimeric complex with Elongin B (TCEB2) and Elongin C (TCEB3), enhancing the processivity of RNA polymerase II by suppressing transient pausing during transcription. This complex is also involved in diverse cellular processes, including DNA repair and ubiquitination, through interactions with the von Hippel-Lindau (VHL) tumor suppressor protein.

ELOA1 antibodies are widely used in research to study transcriptional mechanisms, protein-protein interactions, and the role of Elongin complexes in diseases such as cancer. Mutations or dysregulation of ELOA1 or its binding partners have been implicated in renal cell carcinoma and other malignancies, often linked to VHL pathway disruptions. These antibodies enable detection of ELOA1 expression levels via techniques like Western blotting, immunohistochemistry, and immunoprecipitation, aiding in the exploration of its regulatory functions in hypoxia response (via HIF-1α stabilization) and tumorigenesis.

Developed for specificity and reliability, ELOA1 antibodies are essential tools in molecular biology and oncology, helping to unravel the complex interplay between transcriptional elongation, cellular stress responses, and cancer progression.

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