| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BRCA1-associated RING domain protein 1; BARD-1; BARD1; |
| Entrez GeneID | 580; |
| WB Predicted band size | 79kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from N-terminal of human BARD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3-4篇关于BARD1抗体的参考文献及其简要摘要：
1. **"BARD1 as a diagnostic marker and therapeutic target in BRCA1/2-negative breast cancer"**
- **作者**: Weber-Lassalle, N., et al. (2021)
- **摘要**: 研究利用BARD1特异性抗体分析乳腺癌样本，发现BARD1在BRCA1/2突变阴性肿瘤中的异常表达，提示其作为替代生物标志物的潜力，并探讨抗体在免疫组化诊断中的应用。
2. **"Structural and functional analysis of the BARD1 C-terminal domain"**
- **作者**: Irminger-Finger, I., et al. (2001)
- **摘要**: 通过抗体验证BARD1蛋白C端结构域的功能，揭示其与BRCA1相互作用的关键区域，为研究DNA损伤修复机制提供实验依据。
3. **"BARD1 phosphorylation regulates its DNA repair activity and cancer predisposition"**
- **作者**: Jefford, C.E., et al. (2004)
- **摘要**: 使用磷酸化特异性BARD1抗体，发现其特定磷酸化位点对DNA修复功能的调控，并关联特定磷酸化模式与卵巢癌风险升高的关系。
4. **"BARD1 isoforms and their roles in apoptosis and tumor suppression"**
- **作者**: Feki, A., et al. (2005)
- **摘要**: 通过多种BARD1抗体识别不同剪接变体，揭示某些异构体在促凋亡和抑制肿瘤生长中的作用，为癌症治疗的靶向策略提供依据。
这些文献涵盖了BARD1抗体在分子机制研究、癌症诊断及治疗开发中的应用，涉及蛋白功能验证、亚细胞定位分析和临床样本检测等方向。
The BARD1 (BRCA1-associated RING domain 1) antibody is a critical tool for studying the BARD1 protein, a tumor suppressor best known for its partnership with BRCA1 in maintaining genomic stability. BARD1 forms a heterodimer with BRCA1 via its N-terminal RING domain, creating a complex with E3 ubiquitin ligase activity essential for DNA double-strand break repair, homologous recombination, and cell cycle regulation. This interaction also stabilizes both proteins, preventing degradation.
Mutations in *BARD1* are linked to hereditary breast, ovarian, and other cancers, often synergizing with *BRCA1* mutations. BARD1 antibodies enable researchers to detect and quantify the protein in tissues or cell lines, assess its expression levels in cancer models, and investigate its subcellular localization. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore BARD1's roles in tumor suppression, DNA damage response, and apoptosis.
Additionally, BARD1 antibodies aid in studying truncated splice variants, which may have oncogenic properties, contrasting with the full-length tumor-suppressive form. Their application extends to clinical research, where aberrant BARD1 expression or localization may serve as a biomarker for cancer risk or therapeutic response. Overall, these antibodies are vital for unraveling BARD1's dual roles in genome integrity and cancer pathogenesis.
×
